| BackgroundUlcerative colitis (UC) is a chronic inflammatory colonal disease. Theclinical manifestations include abdominal pain, diarrhea and bloody purulentstool. UC is often characterized by periods of spontaneous remission andsubsequent relapse, consequently, the life quality of UC patients is very poor. Asthe disease develops in some critically ill patients, it is likely to progress intocolorectal cancer(CRC), and patients suffering from UC are at a higher risk ofcanceration than the ordinary population, while there is a lack of relativelyspecific marker capable of indicating UC patients’ caceration risk. Mc serialmonoclonal antibodies are highly involved in colon cancer. Two of the mostspecific and sensitive Mc antibodies, Mc3and Mc5, have been wellcharacterized in CRC. However, their roles in UC related canceration remainunclear. Therefore, Mc3and Mc5were detected in this research for itsexpression status in normal, UC, UC-accompanied intraepithelial neoplasia and sporadic CRC, analyzed for its expressional trends in the course of ulcerativecolitis-intraepithelial neoplasia-canceration, and explored for its potentialclinical significance.MethodBiopsies from300patients at Xijing Hospital, Fourth Military MedicalUniversity from year2006to2011were recruited in this study. Among them,66were diagnosed with UC,52were diagnosed with UC-accompaniedintraepithelial neoplasia,50were diagnosed with CRC,50were normal and82were diagnosed with sporadic intraepithelial neoplasia(IN). Mc3and Mc5wererespectively measured, via immunohistochemical staining, for its expressionstatus in UC, UC-accompanied intraepithelial neoplasia, sporadic CRC andsporadic IN, coupled with statistical analysis of their expressional changes in thecourse of canceration of UC, as well as comparison between UC-accompaniedIN, CRC and sporadic IN.ResultsImmunohistochemical results indicated that Mc3was almost not expressedin the50of normal case, was positively expressed in30%of the66of UC cases(20/66),48%of the50ulcerative-colitis-accompanied IN cases (24/50),91%ofthe46CRC cases (42/46) and84%of the82sporadic IN cases (69/82). χ2testfound Mc3to be statistically differently expressed in normal cases, UC cases,UC-accompanied IN cases and CRC cases （P＜0.05）; and significantlydifferently expressed in UC-accompanied IN cases and sporadic IN cases （P＜0.05）. In the light of the high/low levels within IN and good/moderate/poordifferentiation within CRC, Mc3was further analyzed for its expressional trends in low intraepithelial neoplasia (LIN), high intraepithelial neoplasia (HIN),well-differentiated cancer (WD cancer), moderately-differentiated cancer (MDcancer) and (poor-differentiated cancer (PD cancer), which found that Mc3waspositively expressed in67%of6UC-accompanied HIN case (4/6),45%of44UC-accompanied LIN cases (20/44),94%of18PD cancer cases (17/18),92%of13MD cancer cases (12/13),87%of15WD cancer cases (13/15),75%of40simple LIN cases (30/40), and93%of42simple HIN cases (39/42). Statisticalanalysis demonstrated the significant differences among low, medium and highgrade differentiated CRC（P＜0.05）. In addition, significance also found in highand low grade differentiated sporadic IN（P＜0.05）. There is significantdifference in UC with high and low grade differentiated IN（P＜0.05）.Immunohistochemical results indicated that Mc5was positively expressed in29%of the66of UC cases (19/66),46%of the52UC-accompanied IN cases(24/52),89%of the47CRC cases (42/47) and79%of the82sporadic IN cases(65/82). χ2test found Mc5to be statistically differently expressed in UC cases,UC-accompanied IN cases and CRC cases （P＜0.05）; and significantlydifferently expressed in UC-accompanied IN cases and sporadic IN cases （P＜0.05）. Mc5was positively expressed in46%of46UC-accompanied LIN cases(21/46),50%of3UC-accompanied HIN cases (3/6),100%of18PD cancercases (18/18),85%of13MD cancer cases (11/13),81%of13WD cancer cases(13/16),75%of40simple LIN cases (30/40), and83%of42simple HIN cases(35/42). Statistical analysis illustrated the significant differences among low,medium and high grade differentiated CRC（P＜0.05）. In addition, significancealso found in high and low grade differentiated sporadic IN（P＜0.05）. However,there is no significant difference found in UC with high and low gradedifferentiated IN（P＜0.05）. ConclusionIt was found in our research that Mc3and Mc5were both incrementallypositively expressed along UC, UC-accompanied IN and CRC, as well as alongsporadic IN and WD, MD and PD cancers, which implied an key role that Mc3and Mc5might play as the disease progressed from UC into CRC. |
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