The Investigation Of Radioiodination Labeling Methods And Quality Control For Mouse-Anti-Muc4 Monoclonal Antibody

Objective：To investigate the radioiodination labeling methods includingselecting the optimum conditions, and performing quality control, all ofwhich are a basis for further realizing the biological behaviors in vitro andpotential strategies for Radioimmunoimaging （RII） and Radioimmunotherapy（RIT） of Pancreatic cancer.Methods：This study describes a fast method for radiolabeling ofAnti-Muc4 with Na¹²⁵I to construct molecular probe——¹²⁵I-Mouse-Anti-Muc4, in which both of NBS and Iodogen were used as oxidizing agents.When other conditions are invariable, the reaction temperature, time, ratio ofNa125I/Mouse-Anti-Muc4,Iodogen/Mouse-Anti-Muc4 and NBS/Mouse-Anti-Muc4 are shifted in order to obtain better conditions. The radiolabelingwas analyzed by thin paper chromatography. Then the radiolabeling productswas eluting by the PD-10 columns, collecting protein peak pipe, deterringradioactive and the radiochemical purity. The stability of the radiolabelingcompounds was tested in vitro under different conditions.Results：The radiolabeling ratio of the labeled compound produced bythe Iodogen and NBS methods were about（81.3±0.97）%,（50.7±0.79）%,respectively. The Iodogen is the ideal oxidizing agents. It was the bestcondition for labeling, the ratio of Na¹²⁵I/Mouse-Anti-Muc4 was 1.5,Iodogen/Mouse-Anti-Muc4 was 10, with 15 min reaction time at roomtemperature （25⁰C）. The radiochemical purity was（95.6±1.26）%. After 48 hours stored in NS or 1% fetal bovine serum, the radiochemical purity was tomaintain about 90%.Conclusion：The molecular probe——¹²⁵I-Mouse-Anti-Muc4 wassuccessfully constructed for the first time; Iodogen as an ideal method forlabeling Mouse- anti-Muc4 with¹²⁵I, it can get ideal radiolabeling ratio andradiochemical purity. It can affect the radiolabeling results that differentreaction temperature, time and ratio of Na¹²⁵I / Mouse-Anti-Muc4, Iodogen/Mouse-Anti-Muc4 and NBS/ Mouse-Anti-Muc4. The radiolabeling ratio washigh, and the labeled compounds were stable. But the immune active,biological behavior and potential strategies for Radioimmunoimaging （RII）and Radioimmunotherapy （RIT） of Pancreatic cancer deserves furtherassessment.