The Mechanism Of Human Vascular Endothelial Cell Inflammatory Damage By Urate

OBJECTIVE:To observe the role of different concentration of urate in the expression of human vascular endothelial cell inflammatory injury associated factors.METHODS:A11experiments were performed with human vascular endothelial cells cultured in vitro. According to the concentration of urate in the culture medium, stimulations were divided in to five groups:H2(720μmol/L)、H1(540μmol/L). M(420μmol/1I)、L1(300μmol/L)、L2(180μmol/L).Control group is non-urate. Different concentrations of urate were used to stimulate vascular endothelial cells for24hours. The levels of MCP-1、ICAM-1and VCAM-1in the supermantants of cell cultures were measured by enzyme linked immunosorbent assay(ELISA). Meanwhile, the expression of MCP-1、ICAM-1and VCAM-1mRNA were tested by using RT-PCR.RESULTS:①MCP-1:MCP-1expression increased in H2(720μmol/L) and H1(540μmol/L) groups compared with the control group(P<0.05). MCP-1expression increased in H2(720μmol/L) compared with M(420μmol/L) and H1(540μmol/L). The expression of MCP-1mRNA increased in H2(720μmol/L)、Hl(540μmol/L) and M(420μmol/L) groups compared with the control group(P<0.05).②ICAM-1:ICAM-1expression increased in H2(720μmol/L) and H1(540μmol/L) groups compared with the control group and M(420μmol/L)(P<0.05). The expression of ICAM-1mRNA increased in H2(720μmol/L)、Hl(540μmol/L) and M(420μmol/L) groups compared with the control group(P<0.05).③VCAM-1:There was no difference in the expressions of VCAM-1and VCAM-1mRNA between the control group and other groups(P>0.05)CONCLUSIONS:Urate can activate endothelial cells directly, cause them to release inflammatory cytokines and induce inflammatory response.