Quantitation Detection Of T Lymphocyte Subsettings, Serum IL-17and Their Significance In CHB Patients

Object: We detected the peripheral T lymphocyte subsettings and the expression of serum il-17in chronic hepatitis(CHB) patients, HBV careers and healthy control to evaluate the immune status and investigate the relationship between them and HBVDNA levels, HBeAg titers, liver damage as well as the index of liver cirrohosis in HBeAg-negtive and HBeAg-positive CHB,repectively,in order to assess the change and clinical significance of them in CHB patients.Method:81CHB,22HBV carriers and20healthy blood donors were included in our present study, and there were36HBeAg-positive chronic hepatitis(CHB) patients and45HBeAg-negtive chronic hepatitis(CHB) patients. HBeAg-negtive CHB patients and HBeAg-positive CHB patients were stratified by HBVDNA levels and HBeAg status, respectively. HBeAg was detected by chemiluminescence method; The serum HBV viral loads were detected by the real-time quantitative PCR; The peripheral T-lymphocyte subsets (the percentages of CD3+, CD4+, CD8+cells and the ratio of CD4+/CD8+) in these patients were determined by flow cytometry; Serum levels of IL-17was measured by using the commercial ELISA assay.Result:1. The percentages of CD3+, CD4+T lymphocytes in peripheral blood and CD4+/CD8+ratios in Chronic Hepatitis B(CHB) patients and HBV carriers were significantly lower than those in control patients (all P<0.05or0.01); In contrast, the percentages of CD8+T lymphocytes showed the opposite trend, and there were also statistically significant (p<0.05or0.01)).2. HBV replication level had a positive correlation with the CD8+T lymphocytes and negative correlation with the ratio of CD4+/CD8+T lymphocyte in HBeAg negtive patients (P<0.05). However, there was no corelational relationship between the HBV DNA level and the CD3+T, CD4+T, CD8+T, the ratio of CD4+T/CD8+T and in HBeAg positive patients, nor did with the HBVDNA levels and the levels of IL-17in the HBeAg negative and HBeAg positive patients,repectively (P>0.05)3. There was no corelational relationship between the HBeAg titers and the CD3+T, CD4+T, CD8+T, the ratio of CD4+T/CD8+T and the levels of IL-17in HBeAg positive patients.4. There was no corelational relationship between the IL-17and ALT,TBil in the HBeAg negative and HBeAg positive patients,repectively,however,there is a positive correlation with IL-17and A/P (P<0.05)Conclusion:1. Cellular immune function decreased and immune dysregulation in various clinical types of chronic HBV infected patients. The elevated serum levels of IL-17was obtained in the CHB patients,suggesting its; potential role in the immune activation of CHB2. HBV DNA level is positively correlated with the cellular immune status in the patients with HBeAg negative chronic hepatitis B, howerver, there was no corelational relationship between the HBV DNA level and the T lymphocyte subsettings in HBeAg positive patients, nor did with the HBVDNA levels and the levels of IL-17in the HBeAg negative and HBeAg positive patients, repectively.3. There was no corelational relationship between the HBeAg titers and the the T lymphocyte subsettings and the levels of IL-17in HBeAg positive patients.4. There was no corelational relationship between the IL-17and ALT,TBil in the HBeAg negative and HBeAg positive patients,repectively,however,there is a positive correlation with IL-17and A/P, suggesting IL-17may be related with the liver fibrosis of CHB.