A Study On Relationship Between Familial Clustering Of Hepatocellular Carcinoma And Such Factors As Polymorphism Of XPD Genes,Etc.in Guangxi Popution

Objective:To study the relationship between genetic polymorphisms of XPD751,external exposure factors and the phenomenon of hepatocellular carcinoma family-clustering in Guangxi.Methods:To the124cases from PLC high incidence families and129from control families, relevant external exposure factors of PLC were collected by the epidemiological questionnaires in the high risk area of Guangxi, at the same time10ml peripheral blood (EDTA anticoagulant and non-anticoagulant each5ml) were collected, the infection rate of HBV in serum of non-anticoagulant were tested and the serum of EDTA anticoagulant were used to test the genotype of XPD751. Distribution frequency differences of the genotypes and alleles of XPD751between PLC high incidence families and control families in Guangxi was tested by Chi-square test.The non-condition logistic regression were used to analysis the major high risk factors of the familial susceptibility to PLC, then analyzed the relationship of gene-external exposure factors interaction.Results:There were no statistically differences in the general demographic characteristics(age, sex, marriage, genetic connection and so on) between cases and controls, respectively(P>0.05).The polymorphism of XPD751of control families were in accordance with the Hardy-Weinberg Equilibrium among the controls(P>0.05).Among the high risk family group and control group,37.1%and11.6%respectively were HBsAg positive in serum, the difference was significant(χ2=22.414, P<0.001), OR (95%CI) was4.482(2.340～8.586).The XPD751Lys/Gln genotype frequencies, in high incidence of family group and the control family group, were21%and7.8%respectively. The difference were significant between two groups(χ2=9.048, P=0.003).The G alleles frequencies,in two groups,werell.3%and3.9%respectively.The difference were significant between two groups(χ2=10.008, P=0.002). Application of non-conditional logistic regression model of genotype of XPD751, HBV infection, corn intake, water type, smoking history, drinking history and analysis found:the major risk factors of the PLC family-clustering in Guangxi is corn-based food, infection of HBV, carrying genotype of XPD751Lys/Gln.The interaction analytical results of the family-clustering incidence of the major risk factors:To analysis the interaction that the XPD751gene associated with HBV infection and the staple maize respectively in hepatocellular carcinoma. It showed that XPD751gene combined with HBV infection might have synergistic action on the susceptibility of family-clustering of Guangxi (P=0.039); XPD751gene combined with corn-based food might no synergistic action on the susceptibility of family-clustering of Guangxi (P=0.948).Conclusion:The polymorphism of XPD751gene and the PLC family-clustering of Guangxi may be relevant. The major external exposure factors were the faction of HBV and corn-based food. On the statistical significance, XPD751gene may have interaction with HBV,while may be no association with the faction of corn-based food in the PLC family-clustering in Guangxi, which need more interactive cases to proof.