Effects Of Oncaspar On Proliferation And Apoptosis In Human Non-hodgkin’s Lymphoma Cell Line And The Preliminar Mechanism

Objective:To investigate the inhibiting and apoptosis of Oncaspar againsted Jeko-1, Raji, Jurkat of human non-hodgkin’s lymphoma Cells line in vitro,and its possible mechanism of induced apoptosis was explored, that would provide experimental evidence and the theoretical basis for clinical application.Methods:Jeko-1, Jurkat, Raji cells were treated with different concentrations of Oncaspar for24h and48h, and proliferations of cells were evaluated by MTT assay; Cells apoptosis change and the cells cycle distribution were examined by flow cytometry(FCM); cell morphological changes in fluorescence microscope after dyeing orange (AO) were observed, The real-time quantitative PCR (RQ-PCR) was used to detect the expression of Bcl-2, Bax, Fas.The statistical analysis was executed by SPSS13.0software, using t-test and analysis of variance for statistical, statistically significant level was"a=0.05".Results:①The MTT method shows that Oncaspar could significantly inhibit the proliferations of Jeko-1, Raji, Jurkat cells, there were differences between different time and concentration groups, and the Oncaspar treatmenting48h group of inhibition effect was significantly higher than24h group0.05). ②Three lymphoma cells’apoptosis and cycle distribution were detected by FCM, compareing with the control team, and medicine group cells apoptosis rate was increasing, and cell cycle distribution changed significantly by blocking cells at G0/G1phase.③With the inverted microscope, the morphological changes of apoptotic Jeko-1, Raji, jurkat cells in Oncaspar groups were observed. After AO dying, fluorescence microscope also showed obviously cells apoptosis characteristics.④Oncaspar could inhibit expression of Bcl-2and up-regulation of Bax, Fas expression on Raji cells, and significantly suppress CyclinDl expressions on Jeko-1cells by detecting with RQ-PCR.Conclusion:①Oncaspar significantly inhibit the proliferation of non-hodgkin’s lymphoma cell lines Jeko-1, Raji, and Jurkat cells, it show a certain time/dose-dependent,The most sensitivities cell to Oncaspar were Jeko-1cells.②Oncaspar mainly induced Jeko-1, Raji, and Jurkat cells apoptosis, but not necrosis.③Oncaspar influence distribution of the cell cycle, and blocking it at G0/G1phase.④Oncaspar down-regulation Bcl-2of Raji cells, and CyclinDl of Jeko-1cells expression and up-regulation Bax, Fas expression of Raji cells, it may be one of the mechanism of induced-apoptosis and cell cycle blocking.⑤Oncaspar through the apoptosis mechanisms to inhibit th growth of cells in vitro, its apoptosis way may include the mitochondria and death receptor.