The Expression Of AKR1B10in Human Hepatocellular Carcinoma Under BI-Exposure Of Hepatitis B Virus And Aflatoxin B1, From Guangxi, China

Objective:through studying the expression level of AKR1B10under the bi-exposure condition of hepatitis B virus (HBV) and aflatoxin B1(AFB1), to explore the relationship between AKRIB10, under bi-exposure condition, and the occurrence and development of hepatocellular carcinoma (HCC).Methods:59cases, from different district of Guangxi province, were categorized into HBV/AFB1-bi-exposure-HCC group (experimental group A),16cases; HBV (+)/AFB1(-) group (control group B),15cases; HBV (-)/AFB1(+) group (control group C),15cases; and HBV (-)/AFB,(-) group (control group D),13cases, according to the exposure condition of HBV and AFB1. And for normal controls,20cases of hepatic hemangioma liver resection and liver transplant donor hepatic tissue were selected.By means of RT-PCR and Western bolt, the expression condition of the protein were tested.Results:The Reverse Transcriptase PCR result showed that the mean expression level of AKR1B10mRNA in HBV/AFB1-related group was significantly higher than HBV-related group (1.39±0.69vs.0.88±0.28, p=0.002), AFB1-related group (1.39±0.69vs.1.03±0.42, p=0.032) and nonHBV/AFB1-related group(1.39±0.69vs.0.58±0.14, p<0.001). The AFB1-related group also showed significantly higher expression level of AKR1B10mRNA than nonHBV/AFB,-related group (1.03±0.42vs.0.58±0.14, p=0.011). The difference was not statistically significant between HBV-related group and nonHBV/AFB1-related group. In the Western-blot result, the mean expression level of AKR1B10protein in HBV/AFB1-related group was significantly higher than HBV-related group (2.79±1.30vs.2.03±0.98, p=0.045) and nonHBV/AFB,-related(2.79±1.30vs.1.45±0.49, p<0.001) but not than AFB1-related group(2.79±1.30vs.1.16±0.31, p>0.05). The difference was not statistically significant among HBV-related group, AFB1-related group and nonHBV/AFB1-related group, although AFB1-related group showed higher expression level of AKR1B10protein than other two groupConclusion:In hepatocellular carcinoma, especially in the aflatoxin B1-related hepatocellular carcinoma, the high expression of AKR1B10is a frequent biological event. Overexpression of AKR1B10may be involved in the carcinogenic process of AFB1, and it may be one of the potential clinic values of AFB1-related HCC molecular biomarkers.