Changes Of Substance P、Calcitonin Gene-related Peptide And Acetylcholine After Acute Myocardial Ischemia In Diabetic Rats

Objective This study will research the expression characteristics of substance P （SP）、calcitonin gene-related peptide (CGRP) and acetylcholine (Ach) in cardiac ganglion cell、atrialmuscle and ventricular muscle after acute myocardial ischemia（AMI）in diabetic rats,to observethe biological characters of SP、CGRP and Ach that cardiac ganglion and myocardium secrete inacute myocardiac ischemia. And its impact on diabetes.Explore the influence that cardiacganglion and myocardium secrete SP、CGRP and Ach in diabetes. For further reveal mechanism,the influence of cardiac function、 structure、 the electric physiology and pathology indiabetes,provides the basis.Methods The study was carried out in a series of experiments:1、By histopathological technique、NISSL body dyeing、hybridization in situ andimmunofluorescence technique,observing the distribution and morphological characteristics ofcardiac ganglion, to clear the type of the ganglion,observe the expression and coexpression ofsubstance P、CGRP and acetylcholine’s in cardiac ganglion cell.2、Establish acute myocardial ischemia model，This research,by immunofluorescence andenzyme linked immunosorbent assay technique，observe the expression of substance P、CGRPand acetylcholine in cardiac ganglion cell from molecular level and protein expression levelfollowing acute myocardial ischemia,to probe into the function and mechanism of cardiacganglion in acute myocardiac ischemia.3、Establish diabetes acute myocardial ischemia model,this research,by enzyme linkedimmunosorbent assay, observe the expression of substance P、CGRP and acetylcholine incardiac ganglion cell of diabetic rats following acute myocardial ischemia from proteinexpression level,in order to understand the function and mechanism of cardiac ganglion ofdiabetic rats in acute myocardial ischemia.Results1、The cardiac ganglion is mainly distribute in heart adipose tissue、arounding the greatvessels、 the outer membrane of atrium and atrial muscle,and some one in ventricularmuscle.There are coexpression of neurons of SP and Ach、CGRP and Ach in heart.2、①Compared with the controls，SP content were significantly up-regulated in ischemiaatrial organization at30min of CAO (P<0.05)，while have raised trend in ischemia ventricularmuscle；②Compared with the controls，CGRP content were significantly up-regulated inischemia atrial organization and ischemia ventricular muscle at30min of CAO(P<0.05)； ③Compared with the controls，Ach content were significantly up-regulated in ischemia atrialorganization and ischemia ventricular muscle at30min of CAO(P<0.05).3、①SP and CGRP’s expression were up-regulated in atrium and ventricle of diabetic ratsat30min of CAO than control group,while the expression of CGRP in ventricular increaseddramatically.But the increase rate significantly lower than the normal rats.②SP and CGRPcontent were significantly up-regulated than control group in atrium and ventricle of diabeticrats at30min of CAO after giving small doses of capsaicin (20mg/kg).And its increase ratesignificantly higher than diabetic rats.Conclusions1、The cardiac ganglion is mainly distribute in heart adipose tissue、arounding the greatvessels、 the outer membrane of atrium and atrial muscle,and some one in ventricularmuscle.There are coexpression of neurons of SP and Ach、CGRP and Ach in cardiac ganglion.2、Acute myocardial ischemic induce SP expression up-regulation in atrial muscle，CGRPexpression up-regulation in atrial muscle and ventricle muscle.3、Diabetes cause SP and CGRP expression down-regulation in myocardium when acutemyocardial ischemia.4、Small doses of capsaicin can partly reverse the effect that diabetes cause SP and CGRPexpression down-regulation in myocardium when acute myocardial ischemia.