The Expression Of Telomere Protection Protein Tin2 And Pot1 Of Patients With Myelodysplatic Syndromes(mds)

Objective1. To study the expression of telomere protection protein TIN2 and POT1 at the mRNA andprotein levels;2. To explore the relationship between the abnormal expression of telomere protectionprotein TIN2 and POT1 and the pathogenesis of myelodysplatic syndromes(MDS);3. To analyze the features of bone marrow cytomorphology of patients with myelodysplaticsyndromes (MDS).MethodsSelecting 51 patients with myelodysplastic syndrome (MDS) and 10 normal controls for thestudy, as the following detections:1. The expression of TIN2 and POT1 genes at the mRNA levels were detected by real-timefluorescence quantitative PCR;2. The expression of TIN2 and POT1 at the protein levels were detected by western blot;3. The features of bone marrow cytomorphology of patients with MDS were detected byWright -Giemsa staining;4. The chromosome karyotype of patients with MDS were detected by R-banding.ResultAccording to the 2008 World Health Organization (WHO) diagnosis and classificationschemes, all MDS patients were divided into refractory anemia(RA), refractory anemia withringed sideroblasts(RARS), refractory blood cells decreased with multilineage dysplasia(RCMD),refractory anemia with excess blasts-1(RAEB-1), refractory anemia with excess blasts-2(RAEB-2), unclassified MDS , MDS with simple 5q-.1. At the mRNA levels, the expression of TIN2 in RA/RARS/RCMD/MDS-U group,RAEB-1 and RAEB-2 group was significantly higher than the controls(P<0.05).There was no significant difference between RA/RARS/RCMD/MDS-U group and RAEB-1, RAEB-2.Theexpression of TIN2 in RAEB-2 group was significantly higher than the RAEB-1 group(P<0.05).The expression of POT1 in RA/RARS/RCMD/MDS-U group, RAEB-1 and RAEB-2 group wassignificantly lower than the controls(P<0.05). There was no significant difference betweenRA/RARS/RCMD/MDS-U group and RAEB-1, RAEB-2. There was no significant differencebetween RAEB-2 group and RAEB-1 group.2. At the protein levels, the expression of TIN2 in RA/RARS/RCMD/MDS-U group,RAEB-1 and RAEB-2 group was significantly higher than the controls(P<0.05). There was nosignificant difference between RA/RARS/RCMD/MDS-U group and RAEB-1.The expression ofTIN2 in RAEB-2 group was significantly higher than the RA/RARS/RCMD/MDS-U andRAEB-1 group (P<0.05). The expression of POT1 in RA/RARS/RCMD/MDS-U group,RAEB-1 and RAEB-2 group was significantly lower than the controls(P<0.05). The expressionof POT1 in RA/RARS/RCMD/MDS-U group was significantly lower than the RAEB-1 andRAEB-2 group(P<0.05). There was no significant difference between RAEB-1 and RAEB-2group.3. 76 cases of MDS bone marrow cell morphology observation and analysis found that themost common abnormalities of granulocyte series are the P-H abnormal myeloid cells (21 cases,27.6%) and the following part of the grain cytoplasm granules decreased (20 cases, 26.3%); themost common abnormalities of erythrocyte series are the old nuclear juvenile pulp (34 cases,44.7%), polychromatic dye (34 cases, 44.7%), and hollow area is too large (29 cases, 38.1%);megakaryocytic abnormalities mainly in the small megakaryocytes (14 cases, 18.4%).4. The chromosome karyotype of 51 patients with MDS,18 cases of MDS found theabnormal chromosome karyotype, the most common abnormalities are +8(7 cases,38.9%) and-7(4 cases,22.2%)Conclusion1. The abnormal expression of TIN2 and POT1 may be involved in the regulation oftelomere dynamics of MDS patients,the regulatory mechanism may be related to the telomerelength;2. The abnormal expression of TIN2 and POT1 may be related to the pathogenesis of MDS,the role in different diseases and different stages of the disease may be different;3. Myelodysplastic syndrome (MDS) of the diagnosis depends on bone marrowdyshaematopoiesis, but dyshaematopoiesis is not necessarily the MDS, need to combine cytogenetics, bone marrow biopsy and other laboratory tests. Also, it is very important to excludeother causes of dyshaematopoiesis.