Cyclopamine To MC Cell Proliferation Of Apoptosis Influence

Objective: The Hedgehog signal channel blockers (Cyclopamine) to MC cell proliferation ofapoptosis influence.Methods: Taking the cryopreservation of GES-1 cells which were revived and passaged. Until80% cells mixing, the cells were placed into 6mm petri dishes after cell counting. Adding the0.01mol/L solution of MNNG into GES-1 cells which were growing strongly,the finalconcentration is 2310-5mol/L. Replacing the culture solution without MNNG after the cellscultured 24 hours without sunlight. The treatment of the GES-1 MNNG cells (named for MCcells, growth properties change, present the tumor cells and the growth of the characteristics ofmorphology.) . Take logarithm growth cells into ring and MC and negative control group wasdealing group, with 1, 5, 10μmol/L ring and amine processing MC cells, respectively in 24, 48,72 h by MTT method to detect after its growth inhibition rate; Flow cytometry and 10μcontrolgroup was testing mol/L ring of the cell cycle and amine influence.Result: 1, 5, 10μmol/L concentration of cells of survival rates were 50.03 + /-14.02) %, 49.76 +/-14.14) % and 37.87 + /-19.53) %, more obvious differences between groups statisticalsignificance (P < 0. 001). Operation flow cytometry detection, the ring and the concentration of10μamine mol/L, intervention 24 h after a significantly higher proportion G1 phase cells. Thecontrol group, 10μmol/L concentration of the G1 phase cells percentage respectively 33.94%,49.83%; The cell apoptosis rate in the control group and 10μmol/L concentration theexperimental group were 1.34%, 7.09%.Conclusion: Ring and amine inhibitory effects of the cells in MC limitation andconcentration-response dependencies. Ring and amine can restrain MC cell proliferation capacityand induction of apoptosis MC function, its mechanism and inhibit the Hedgehog signalpathway.