Influence Of Oral Interventions On Serum Inflammatory Marker In Rats

ObjectiveWe established chronic periodontitis(CP) with or without atherosclerosis(As) model in rats, simulating all kinds of oral interventions (including natural process, tooth extractions, systemic antibiotics, scaling and root planning) to explore to study the effect of oral interventions on atherosclerosisMethodssix-week-old, male SD rats were fed regular chow for2weeks. Then they were randomly divided into four groups:control group(group A), atherosclerosis group(group B), periodontitis group(group C), periodontitis and atherosclerosis group(group D). The rats for model of atherogenesis were fed a high-fat diet to induce the accumulation of lipid deposits in the carotid artery. The CP group received silk ligatures and wire around their maxillary premolars followed by an application of a periodontal pathogen, Porphyromonas gingivalis(P.g), to induce periodontitis. The As+CP group were received the above two methods to induce periodontitis and atherogenesis. Then simulating all kinds of oral interventions divide group C and D into natural process(C1/D1), scaling and root planning group(C2/D2), systemic antibiotics group(C3/D3) and tooth extraction group(C4/D4).The pathological lesion of carotid artery plaque was stained with hematoxylin and eosin. Serum high-sensitivity C-reactive protein, interleukin-6levels were evaluated before oral interventions and1weeks after interventions by ELISA. All animals were scarificed after14weeks.Results1. In whole, the serum hsCRP、IL-6levels of group B and C (except group C3,the number<3) were significantly elevated after oral interventions compared with that of before (P<0.01); after oral interventions, the serum hsCRP、IL-6levels of group B and C4gradually increased and had significant difference between twice sample time-points (P<0.01); While the serum hsCRP levels of group C1was just have significant difference between twice sample time-points (P<0.01); Compared with group A, the levels of serum hsCRP、IL-6of group B and C were significantly increased after oral interventions (P<0.01); Compared with group B, the levels of group C1and C2were lower than that of group B after the second oral intervention (P<0.05), while the level of group C4was significantly higher than that of group B after twice oral interventions (P<0.05).2. In whole, the serum hsCRP、IL-6levels of group B and D (except group D1and D4,the number<3) were significantly elevated after oral interventions compared with that of before (P<0.01); after oral interventions, the serum hsCRP、IL-6levels of group B、D2and D3gradually increased and have significant difference between twice sample time-points (P<0.01); Compared with group A, the levels of serum hsCRP、IL-6of group B and D were significantly increased after oral interventions (P<0.01); Compared with group B, the serum hsCRP、IL-6levels of group D2and D3were all significantly higher than that of group B after twice oral interventions (P<0.01).Conclusions1. In systemically healthy rats with periodontitis, either tooth extractions on the inflammatory stage or permanent periodontitis could increase the risk of atherosclerosis. Periodontal therapy (scaling and root planning) might reduce the risk of atherosclerosis.2. In rats with periodontitis associated with systemically cardiovascular diseases or systemic inflammation, direct oral interventions may increase the risk of carotid atherogenic plaque progression, periodontal basic therapy (scaling and root planing) might especially increase the risk of atherosclerosis plaque progression. To avoid the cardiovascular events, we presumed that the proper time of periodontal basic therapy on patients with cardiovascular diseases should be carefully considered.