Change Law Of PGD₂,CPA₃ And PAF In The Guinea Pig Of Anaphylactic Shock

Objective To assess the change of LTE₄, PGD₂, CPA3 and PAF in the guinea pig death ofanaphylactic shock, and look for biomarkers to diagnose the anaphylactic shock.Methods①Guinea pigs of either sex were used to establish anaphylactic shock models.②The contents of LTE₄, PGD₂and CPA3, and PAF were detected respectively in urine, plasmaand brain tissues with ELISA kit. The contents of urine creatinine were detected with H ITACHI7170A Automatic biochemical analyzer. The significant biomarkers were selected. The ratio ofthe LTE₄in to the urine creatinine was calculated.③Based on the tests of the electedbiomarkers, to observe the change law of PGD₂, CPA3 and PAF in the guinea pig respectively in0 h, 12 h and 24 h after died of anaphylactic shock.④To observe the effect of PAF-AH inreducing the contents of PAF.Results①There were no difference of the level of LTE₄in urine between experimentalgroup and control group（P＜0.05）, But the ratio of the LTE₄in to the urine creatinine inexperimental group were significantly higher than control group（P＞0.05）. The contents ofCPA3, PGD₂and PAF in experimental group were significantly higher than control group（P＜0.05）.②The level of PAF in 12 h and 24 h after anaphylactic shock were significantly higherthan control group（P＜0.05）, and The level of CPA3 and PGD₂in 12 h after anaphylactic shockwere significantly higher than control group（P＜0.05）.③The level of PAF treated withPAF-AH in advance were significantly lower than treated with normal saline（P＜0.05）.Conclusion①The LTE₄in urine cannot be selected as biomarkers to diagnose theanaphylactic shock if the urine creatinine was not known.②The PGD₂and CPA3, and PAFrespectively in plasma and brain tissues maybe used as biomarkers to diagnose the anaphylacticshock.③The PAF-AH maybe used for clinical treatment.