Identification Of A Novel Transcript Of The AML1 Gene

Objective (1)To clone and identify the full sequence of the novel 5-6-5 transcript of the AML1gene. (2) To discuss the formation mechanism of the 5-6-5 transcript. (3)To statistically analyzethe differences of the 5-6-5 transcript expression in healthy individuals and acute myeloidleukemia patients which were collected in our hematology department.Method (1) The novel 5-6-5 transcript was identified by reverse transcriptase-polymerasechain reaction (RT-PCR) combined with sequencing. (2) By using RNase R digestion, thestructure of the 5-6-5 transcript was deduced. (3) Real-time PCR was performed to detect theexpression level of the novel transcript in 20 healthy individuals and 60 acute myeloid leukemiapatients, and to explore the differences among AML1/ETO(+) AML patients, AML1/ETO(-) AMLpatients and healthy individuals, the nonparametric Kruskal Wallis test was used.Result (1) The novel transcript with 5-6-5 exon alignment existed. (2) The novel transcriptdidn’t possess the polyadenylate tail, and resisted the RNase R digestion. (3) There was nostatistically significant difference observed between the expression of the novel transcript inacute myeloid leukemia (AML) patients and healthy individuals.Conclusion (1) The novel transcript of the AML1 gene was circular RNA, whose exon5 andexon6 were joined head to tail. (2) Its expression in healthy individuals and AML patients wereof no statistical significance.