Study On The Effects Of Celastrol On The Apoptosis And Cell Cycle Arrest Of C6Glioma Cells And Its Mechanism

Objective：To investigate the effects of celastrol on the apoptosis and cellcycle arrest of C6glioma cells and its mechanism.Methods:C6glioma cells were cultured in vitro. MTT was used to assaycellular proliferation. Fluorescence microscope and transmission electronmicroscope were used to evaluate the morphological alterations of C6gliomacells after being incubated with2.56μmol/L celastrol. Flow cytometry wasused to analyze the alterations of apoptosis rate and cell cycle.Results:The inhibitory effects of celastrol on C6glioma cells weredependent on celastrol concentration and incubation time. Typicalmorphological alterations of apoptosis were observed under photo-microscope,fluorescence microscope and transmission electron microscope respectively.Flow cytometry showed that the apoptosis rate increased from1.32%incontrol group to19.34%in celastrol group, and cellular quantity decreasedfrom76.42%to54.52%at G0/G1phase but increased from9.29%to30.50%at G2/M. Western blotting analysis showed that the expression ofapoptosis-related proteins Bcl-2and XIAP were down-regulated, but Bax,Caspase-3and cleaved PARP were up-regulated. Moreover, the expression ofcell cycle regulator p21, p2and cyclin B1were increased, but cdk2wasdecreased.Conclusions:Celastrol could induce C6glioma cell apoptosis and cellcycle arrest via influencing the expression of apoptosis related proteins and cell cycle regulators.