| Gastric cancer is the globally highest incidence and its biologicalmechanism is related to many factors, multiple stages, multiple gene expression,is normal gastric mucosal cell growth and differentiation mode control. It is aserious threat to people’s health and the common digestive tract tumor. Inrecent years the early gastric cancer in China the discovery rate and thediagnosis rate is still low, when diagnosed tend to be advanced stage or late,most gastric lymph nodes have shifted, so radical operation combined therapyis still for the international of choice for gastric and main treatment methods.So the study on diagnosis of early gastric cancer markers and tumor cellmetastasis and recurrence mechanism, to explore the early gastric cancerprediction, early prevention and treatment of tumor cell transfer method, is stillthe international study of gastric cancer basic and clinical Chongzhongzhi isheavy. Therefore, we study on lymph node metastasis of gastric cancer andgastric carcinoma cell miR-451a expression in gastric cancer cells, aims toexplore the mitosis, infiltrative growth correlates with lymph node metastasis,gastric cancer is early detection, early diagnosis and comprehensive treatmentof referential value of molecular biology on levels of markers.With the development of modern molecular biology and tumor immunecell genetic engineering technology’s rapid development, more and moreinternational scientists found that, in the metastasis of tumor cells in miRNAplays an extremely important role mechanism, as the tumor cell metastasisrelated to early discovery and diagnosis and treatment to provide newinternational field of vision. Malignant tumor cell metastasis is a complexbiological process, the number of research results, miRNA in tumor cellsduring the transfer of more complex link. MiR-451a through positive feedback regulation of E2F1, promote their apoptosis induced by activity, inhibition ofc-myc mediated tumor cell proliferative activity, further effectively inhibitmalignant cell mitotic and proliferation kinetics. Objective: throughmiRNA-451a in gastric carcinoma tissue cells in the expression profilinganalysis, and analysis in the gastric cancer lymph node metastasis and thegastric cancer tissues and cells in miR-451a expression differences, on gastricmucosal epithelial cells in the tissue in the process of malignant transformationof cell and molecular mechanisms of action, as of early gastric cancerdiscovery, diagnosis and clinical comprehensive treatment provides importantbasis.Methods: in the experimental group specimens taken from Decemberto2011in2009Jilin University in December Second Hospital General Hospitalof gastrointestinal surgery was performed on47cases of gastric canceroperation patients. Specimens of tissue from the Jilin University secondhospital general hospital pathological tissue specimen database. The controlgroup specimens from Jilin University in second hospitals endoscopic roomand pathology results in return for chronic mucosal inflammation specimens ofpatients with wax block. Note: the experimental specimens withoutmicroscopic pathologic return for intestinal metaplasia in patients. Theexperimental design is divided into five groups. The first group based onspecimens from patients with chronic mucosal inflammation. Second groups ofgastric antrum ministry based in differentiated adenocarcinoma (in operation toremove all perigastric lymph node metastasis negative) experimentalrequirements for carcinoma in situ. Third groups of gastric antrum ministrybased in differentiated adenocarcinoma (the first station that is3,4,5,6groupsof lymph node metastasis as positive material17lymph nodes pathologicalreturn rate of lymph node metastasis in5/17) fourth groups from gastricantrum of differentiated adenocarcinoma (second,1,7,8stations,9groups oflymph node metastasis as positive material17lymph nodes pathological returnrate of lymph node metastasis in13/17) fifth groups from gastric antrum of differentiated adenocarcinoma (third stations2,10,11,12,13,14groups of lymphnode metastasis as positive material15lymph nodes pathological return rate oflymph node metastasis17/17each sample)(primary gastric lesion) is dividedinto two parts, a portion of the sample in the tissue in vitro10min cryopreservedin liquid nitrogen, used for RNA analysis; another part samples preserved informalin solution, fixed paraffin-embedded, used for histopathological analysis.Through real-time quantitative Real-time determination in gastric cancer tissuesand cells in PCR miRNA-451a expression, and concomitant with gastric cancerlymph node range from high to low in gastric carcinomas and benign ulcertissue cells (Department of pathology results in return for chronic mucosalinflammation) in miRNA-451a expression were compared, control andanalysis. Results of the first group (chronic mucosal inflammation patientspecimens) of miR-451a expression was higher in second groups (in situcarcinoma group). Compared to the fifth group (gastric cancer lymph nodemetastasis in gastric cancer tissues and cells in group N3) miRNA-451aexpression level was lower than third, four groups (gastric cancer lymph nodemetastasis of N2group N1). Concomitant with gastric cancer lymph node rangefrom high to low miR-451a in gastric carcinoma tissue expression levelsin78.7%(n=37) in a sample is reduced,21.3%(n=10) in the sampleincreases. MiR-451a relative expression: third groups (group N1lymph nodemetastasis in gastric cancer tissues and cells) was3.5796+0.2819, fourthgroups (group N2lymph node metastasis in gastric cancer tissues and cells)was2.4698+0.5423, fifth groups (group N3lymph node metastasis in gastriccancer tissues and cells) was1.6263+0.7842, and the first group ofinflammatory control group (pathology of chronic mucosal inflammation inreturn for5.5724+0.3611). The fifth group (N3group of lymph node metastasisin gastric cancer tissues and cells) the expression level of miR-451a averagebelow fourth, three group (N2group N1). Change with significant difference(P <0.05).2levels of miR-451a expression in different tumors with a diameterof WHO, histological type, tumor differentiation, showed no significant difference (P>0.05). Conclusion: inflammatory group of gastric mucosa cellsin the expression level of miR-451a is higher than that of in situ carcinoma andmetastasis of gastric cancer primary tumor in the expression level of miR-451a.With the increased level of lymph node metastasis in gastric carcinoma and itscorresponding gastric cancer cells in the expression level of miR-451adecreased. The mechanism may be related to miR-451a positive feedbackregulation of E2F1, promote their apoptosis induced by activity, inhibition ofc-myc mediated tumor cell proliferative activity, further effectively inhibitmalignant cell mitotic and proliferation kinetics. MiR-451a expression withtumor pathological type was not significantly correlated, but the tumor tissueinfiltration depth and distant lymph node metastasis have obvious correlation. Itis revealed that the miRNA-451a for tumor initiation plays an important role,and are associated with tumor development and pathological typing ofcorrelation is still need further research. |
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