Study Of Peripheral Blood MicroRNA331-3p In The Diagnosis Of Gastric Cancer As Molecular Marker

Background and aim:In our previous experiment we have learned that the peripheral blood microRNA331-3p in gastric cancer is significantly higher than that in non-gastric cancer group. This research is to study the clinical possibility of determining peripheral blood microRNA331-3p in the differential diagnosis of gastric cancer and non-gastric cancer through the analysis of expression levels in general patients and the following sensitivity and specificity.Methods:Peripheral blood samples from general population were collected in Sir Run Run Shaw Hospital affiliated Zhejiang University School of Medicine and supernate was isolated. Total RNA was extracted from supernate using mirVanaTM PARISTM Kit and microRNA331-3p expression level was measured using the All-in-OneTM miRNA qRT-PCR. The results combined with the clinical data of patients were analysed.Result:1)The number of patients included is94and the average peripheral blood expression level of miRNA331-3p is-0.32±02.629;2)The included patients can be classified into three groups:gastric cancer group, pre-gastric cancer group and healthy control group, and the number of groups is44,27,20respectively. The average expression level of miRNA331-3p in gastric cancer group(-1.806±2.04) was significantly higher than that in healthy control group(0.417±1.639) and that in pre-gastric cancer group(1.472±2.837)(p<0.05). Also The average expression level of miRNA331-3p in pre-gastric cancer group was lower than that in healthy control group but has no statistically significant difference; 3) With miRNA331-3p≤-1.1in peripheral blood as positive boundary value, the true positive rate is72.73%, the true negative rate is82%.Conclusion:Determination of miRNA331-3p in peripheral blood plays a role in the differential diagnosis of gastric and non-gastric cancer and miRNA331-3p may be a type of molecular markers of gastric cancer in future.