Detection And Genotyping Of Human Papillomavirus In Clinical Warts Samples

The human papillomavirus (human papillomavirus, HPV) can causebenign hyperplasia of skin and mucous membranes, leading to the occurrenceof a variety of skin warts, and malignant disease of genital skin andmucosa.Different types of diseases are caused by different HPV types. HPVtype also have racial and geographic differences.Object：Analysis the distribution of HPV types in the specimens of the skin lesionsof HPV infection patients.Evaluate the sensitivity, accuracy and clinical valueof polymerase chain reaction (PCR)-reverse dot blot hybridization.Basic onthe data we will set up a useful HPV animal models for drug screening.Method：Genotyping cases of HPV DNA of verruca vulgaris and condylomaspecimens of outpatient of our hospital in October2011to April2012using thePCR-reverse dot blot hybridization method to explore the specific HPVinfectious distribution in Jilin area.Result：In15cases of verruca vulgaris specimens, the number of HPV detection is11cases, the rate of detection is73.3%.The HPV types respectively are: HPV17cases, accounting for63.6%of the total detection number; HPV23cases,27.3%, HPV1,2mixed infection2cases, accounting for18.2%.In the25cases of condyloma acuminatum specimens, the number of HPVdetection is23cases, the detection rate is92%. The HPV types respectively are:the monotonous infection in14cases,60.9%, of which5cases of HPV6,accounted for21.7%; HPV11type9cases,39.1%. Mixed infection9cases, 39.1%. Among them HPV6, type11mixed infection in3cases,13%; HPV6,16type mixed infection in2cases,8.7%; HPV6,18type in2cases,8.7%;HPV11,52type in1cases,4.3%; HPV11,53type in1cases (4.3%).Conclusion：Two HPV subtypes were detected in verruca vulgaris specimens, sixsubtypes in condyloma acuminatum specimens. HPV1type make up thehighest frequent infective rate,which is63.6%;HPV11the highest rate incondyloma acuminatum specimens, which is39.1%. We also found the existingof four high risk HPV type,named HPV16,18,52,53in condyloma acuminatumspecimens.The results showed that using PCR-reverse dot blot hybridization methodto detect the HPV subtypes of skin warts specimen has high sensitivity, highdetective rate, which is suitable for clinical application.