Effects Of Poly(ADP-ribose) Polymerase-1Inhibitor AG014699on Growth And Apoptosis Of Human Ovarian Cancer Cell Line SKOV3

BACKGROUD As we known, the human epithelial ovarian careinoma has complex biological characteristics, high mortality rate, and low five year survival rate due to the difficulty of treatment in advanced stage, its low rate of early detection and easy to relapse, which led the epithelial ovarian cancer to the most threaten disease of female genital malignant. Although the great improvement has been made in standard therapy for the ovarian cancer, the survival rate still has not been obviously improvement. So it’s the most important things that a new and useful medicine appears. Poly(ADP-ribose)polymerase1is an important protein in the base excision repair pathway for DNA single strand breaks. This enzyme was first reported in1963by Chambon. Its expression is highly in many tumors. AGO14699is the inhibitor of PARP-1that can dissolve in water. it can inhibit the growth of cancer,for example,breast cancer associated gene-1and2deficient, melanoma, gliocytoma. AGO14699may be the new medicine of treatment the cancer ’can be used in clinic.OBJECTIVE To explore the effects of Poly(ADP-ribose) Polymerase-1Inhibitor AGO14699on the cell proliferation and apoptosis of human ovarian cell line skov3. Maybe can discover a new medicine to used in the patients of ovarian cancer, and improve the survival rate of ovarian cancer patients.METHODS l.MTT Growth inhibition rate of skov3cells was measured with MTT method, at24h、48h、72h of1.O、10、20、50、l00μmol/L, to determine the time and consistency usd in flow cytometery.2. Flow cytometery Apoptosis rate and cell cycle distribution of the skov3cells at5Oumol/L were inspected by flow cytometery (FCM) after24h.RESULTS1. MTT At10,20,50,100μmol/L,AG014699inhibited the growth of human ovarian cancer cells skov3at2448and72h, P<0.05, compared control group. The growth inhibition rate were increased with the growth of medicine’s consistency, especially at24h.2. Flow cytometery At50μmol/L, AGO14699induced the apoptosis of the human ovarian cancer cell line skov3at24h, and changed the cell cycle distribution of skov3cell compared control group,increasd the rate of GO/G1, P<0.05.CONCLUSION AG014699significantly inhibited the growth of ovarian cancer cells skov3and induces apoptosis.