The Effection Of Lapatinib Combination With Cisnlatin On Human Ovarian Cancer Cell SKOV3and Study On Its Mechanism

Objective:To investigate the effection of new targeted therapy drugs Lapatinib Combination with Cisnlatin (DDP) on cell proliferation and apoptosis on human ovarian cancer cell line SKOV3in vitro and to explore its influence on the expression of P-AKT.Methods:Ovarian cancer cell line SKOV3Were cultured in vitro under the irritation of Lapatinib,DDP or the miscible liquids.The inhibition rates were detected by MTT array; the cell cycle of SKOV3cells exposed to drugs was analyzed by flow cytometry; the expression of P-AKT was also examined by flow cytometry.Results:Lapatinib,DDP obviously inhibited the proliferation of SKOV3cells in a time,had the significant diference compared with the control groups (p<0.05); The inhibition rates of Different concentration of lapatinib Combination with Cisnlatin on Human Ovarian Cancer cell SKOV3were higher than single drug group,had the significant diference compared with the control groups (p<0.05),before and after the application of lapatinib, the expression of P-AKT protein has significantly different (p<0.05).Conclusion:Lapatinib Combination with Cisnlatin can obviously inhibit SKOV3cell proliferation and induce cell apoptosis, the mechanism wasclosely related to downregulation of expression of P-AKT, and provides new targets and certain theoretical basis for its application in ovarian cancer.