Expression Of β-catenin In Tooth Germ Development During Cleft Palate Induced By All-trans Retinoic Acid In Mouse

ObjectiveTo investigate the morphologic changes of tooth germ development during cleft palate induced by all-trans retinoic acid(atRA) in C57BL/6J mouse, and to detect the effect of excess atRA on the expression of (3-catenin in tooth germ development.MethodPregnant mice which were devided into experimental group, vegetable oil control group and blank control group at E13d, E14d, E16d respectively, were examined by slight microscopy. Through in immunohistochemistry, the difference of P-catenin expression in the paraffin-embedded sections between normal and defected cleft palate induced by atRA was analyzed in tooth germ development.ResultsDuring E13d to E16d,positive expression of β-catenin was found in the oral epithelium and the dental lamina, became more and more strong stained where cell actively proliferated. The level of the same developmental time was lower than that of the experimental group during all stages. The same expression level of β-catenin occurred both vegetable oil control group and blank control group.Conclusion The changes of β-catenin expression induced by excess atRA in the epithelium-mesenchymal interaction regulating morphogenesis of tooth germ may be related to the cleft palate induced by all-trans retinoic acid, the expression of β-catenin displayed a up-regulation tendency, Which thus interfered with the development of tooth germ.