The Correlational Study Between Omentin-1and The Distribution Of Carotid Atherosclerosis In IgA Nephropathy

Objrctive(1) To identify the distribution constitute of atherosclerosis in the different classification of IgA nephropathy;(2) To identify serum level of omentin-1in patients with IgA nephropathy;(3) To clariy the associaton between serum level of omentin-1and carotid atherosclerosis in patients with IgA nephropathy.MethodsThis study was carried out at the Second Hospital of Lanzhou University between2010and2012. We enrolled randomly150people with IgAN, evaluated their carotid atherosclerosis employing ultrasonography and then divided them into six subgroups, isolated microscopic hematuria(I-H), recurrent gross hematuria(R-GH), massive proteinuria(MP), urinalysis abnormal(U-ab), Hypertension(HT) and end-stage renal disease(ESRD), Height, weight and blood pressure(BP) were measured. Fasting blood sample was collected to determine to biochemical indicators and insulin resistance index(HoMA-IR). Serum omentin-1levels was assessed by with omentin enzyme-linked immunosorbent assay.And20controls were healthy volunteers enrolled in the study.Continuous variables with normal distributions were expressed as mean±SD. Differences in the mean values between groups were compared by One-Way Analysis of Variance. Correlation between serum concentrations of omentin-1and other parameters were studied by Pearson correlation analysis. Moreover, to study whether there was an independent relationship between omentin-1and IgA nephropathy, multiple linear regression was performed.SPSS17.0software was used for statistical analysis. P<0.05was considered statistically significant.Result(1) The eGFR and serum omentin-1were markedly lower and CIMT,24-hour urinary protein quantification and CRP were significant greater in IgA nephropathy patients than in control group (P<0.01、P<0.05)(2) Age, SBp, CIMT, TG, LDL, HDL, FBG, and HOMA-1R were higher and Serum omentin-1levels and eGFR were lower with ESRD compared with the other IgA nephropathy group (P<0.01、P<0.05)(3) CIMT, SBP, DBP,24-hour urinary protein quantification and CRP were significant greater and Serum omentin-1levels and eGFR were lower with H-T patients compared with I-H or R-GH group (P<0.01、P<0.05)(4) CIMT,TC,TG,LDL,CIMT, HOMA-IR,SBP,24-hour urinary protein quantification and CRP were significant greater and Serum omentin-1levels and eGFR were lower with MP patients compared with I-H or R-GH group (P<0.01、P<0.05)(5)24-hour urinary protein quantification significant higher in U-ab group than in I-H or R-GH (P<0.01)(6) Correlation analysis showed that were positive correlations between age, BMI, SBP, UA, TG. LDL, FBG, HOMA-IR, CRP and24-hour urinary protein quantification and CIMT, negative correlations between eGFR、HDL and Serum omentin-1levels and CIMT (P<0.O1、P<0.05)(7) Significant negative correlations were noted between serum omentin-1levels and CIMT, BMI, SBP, TG, LDL, CRP, FBG and HOMA-IR, while eGFR, HDL showed positive associations(P<0.01、P<0.05).In a multiple linear regression using omentin-1as dependent variable, the independent predictors were BMI, SBP, eGFR, LDL, CRP and HOMA-IR (P<0.O1、P<0.05)Conclusions(1) The CIMT was markedly higher in IgA nephropathy patients than in control group, and significant greater with MP, H-T and ESRD patients compared with I-H or R-GH and U-ab group.which suggesting that IgA nephropathy patients (the MP group, HT group, ESRD group) predispose to atherosclerosis compared to control group.(2) In a multiple linear regression using CIMT as dependent variable, the independent predictors were24-hour urinary protein quantification, UA and HOMA-IR, implicating that the non-classical risk factor of eGFR and proteinuria were involved in the occurrence of carotid atherosclerosis in IgA nephropathy.(3) Serum omentin-1levels were lower with IgA nephropathy compared with control group.(4) Serum omentin-1levels were ignificant lower in MP, H-T and ESRD group than in I-H, R-GH or U-ab.(5) Significant negative correlations were noted between serum omentin-1levels and CIMT, In a multiple linear regression using CIMT as dependent variable, the independent predictors were omentin-1, implicating that High Serum level of omentin-1might play a protective role in the occurrence of carotid atherosclerosis in IgA nephropathy.