| Objective: The treatment of spinal cord injury is a hot and difficult problem in thefield of neuroscience. In recent years, mesenchymal stem cells play an important role inthe nerve tissue repair, regeneration and it’s functional reconstruction. Bone marrowmesenchymal stem cells and umbilical cord mesenchymal cells have been used as cellsources for neural repair of spinal cord injury, and show their function in nerveregeneration and the recovery of nerve function, but It’s not satisfactory BMSCs as aclassic source in transplantation, as aging causes the stem cell’s contents and proliferativecapacity to become significantly low. Therefore, finding a new sources of MSCs isnecessary. We have recently obtained MSCs easily from the primitive human umbilicalcord tissue, other advantages are as folows:①easily obtained, easily accessible;②noethical and moral barriers;③isolated Cells morphology, and it’s phenotype is moreuniform;④MSCs content in the umbilical cord is high, cell proliferation capacity isstable and is easy to acquire enough cells within a short period of time;⑤HUCMSCsdon’t express HLA-DR, weak immunogenicity. This study aims to observe the recoveryof neurological function in the rat model of spinal cord injury and its immune rejectionafter transplantation of human umbilical cord mesenchymal stem cells and rat bonemarrow mesenchymal stem cells, and look for a new kind of stem cell for the applicationprospects of clinical, and thus Promote its application in the treatment of spinal cordinjury.Methods:1. Obtain a healthy, full term, Cesarean sectioned fetal umbilical cord. and then by Trypsin digestion method obtain the MSCs from the umbilical cord.2. Select Spraque-Dawley rats (approximately150g of either sex) and under sterileconditions separate the femur and tibia then Separate and purify the rat’s bone marrowmesenchymal stem cells by density gradient centrifugation combined with adherentculture.3. Select40SD rats from the same batch, using randomized number table dividethem into3gorups: group1consisting16SD rats for umbilical cord mesenchymal stemcell transplantation, group2consisting16SD rats for Bone marrow mesenchymal stemcell transplantation and group3consisting8SD rats for PBS. use spinal cord hemisectionsurgery for rats with spinal cord injury and for damaged cords use umbilical cordmesenchymal stem cell transplantation a group of human umbilical cord mesenchymalstem cells transplantation. Bone marrow mesenchymal stem cell transplantation group issame as that of rat bone marrow mesenchymal stem cell transplantation, PBS grouptransplantation is equivalent to that of PBS. Then using BBB scores compare thepost-operative recovery of rats in each group. The levels of1I-2and IL-10in the bloodof each group were detected at different times(1W and2M,and3months after the celltransplantation)to compare the systemic immunological rejection of each group animalsafter transplantation．By using immunohistochemical method, conditions of the biopsiedCD3+cell infiltration can be detected to check the immunological response aftertransplantation.Results:1. Rat’s bone marrow mesenchymal stem cells have the similar growthpattern and cycle with that of a human umbilical cord mesenchymal stem cells, but ratbone marrow mesenchymal stem cells is significantly slower than human umbilical cordmesenchymal stem cells in proliferation and differentiation.2.1w,1M,2M upto3M after transplantation at each point we found that the II-2levels in rat’s blood stem cell transplantation group were significantly higher than PBSgroup, compared with control group II-10level decreased significantly, and thedifferences were statistically significant(P<0.05). 3. The level of IL-2was obviously higher in the BMSCs transplantation group thanthose in HUCMSCs transplantation group lW and2months and3months after the celltransplantation (P<0.05). While IL-10was contrary to the IL-2.4. The quantity of CD3+cells in all groups was much at the acute period (1W aftercell transplantation), but only the quantity of CD3+cells in bone marrow mesenchymalstem cell transplantation group was much larger than that in the other groups at thechronic period3months after cell transplantation (P<0.05).Conclusion: Compared with the rat bone marrow mesenchymal stem cells, thehuman umbilical cord mesenchymal stem cells have higher multiplication potential andcan maintain their strong multiplication potential even after P5, and compared with bonemarrow mesenchymal stem cells, the umbilical cord mesenchymal stem cells have muchLower immunogenicity,hence HUCMSCs transplantation is more favorable for recoveryof spinal cord injury. HUCMSCs play an important value in the treatment of spinal cordinjury. |
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