Study On The Treatment By Chemotherapy Combined With CIK Cells Of Malignant Melanoma

Objective To generate cytokine-induced killer （CIK） cells by inducing lymphocytescells from peripheral blood in vitro, and study the immune phenotype and cytotoxicactivity of CIK; Then evaluate the clinical of the treatment of postoperative MalignantMelanoma by Chemotherapy Combined with cytokine-induced killer cells.Methods The peripheral blood mononuclear cells （PBMCs） were separated byFicoll-Conray density gradient centrifugation. PBMCs were activated with INF-γ, rhIL-2,rhIL-1α and anti-CD3McAb. The phenotype of CIK cells were analyzed by FACS, and thecytotoxic activity were tested by MTT. A total of thirty-seven Malignant Melanomapatients who received six cycles of chemotherapy after radical gastrectomy werefollowed-up. Among of them, twelve patients received additional CIK cells transfusionafter chemotherapy, twenty-five patients received chemotherapy alone. Both of the twogroups using CVD Chemotherapy scheme：DDP20mg/m²D1-5，VCR1.6mg/m²D1-5，DITC800mg/m²D1. The median overall survival time and the median progress-freesurvival time were compared between the two groups by long-rank test. In addition, thechange of T-cell subgroup after CIK cells transfusion was observed in the study.Results①The number of CIK cells was increased significantly during cell culture,reaching the highest on day14to21. The cytotoxic activity of CIK cells to K₅₆₂and Rajiwas highest on day14,（76.65±16.78）%and （75.32±15.61）%respectively.②NK cells,CD3⁺CD56⁺T cells, CD4⁺T cells, CD3⁺T cells and the ratio of CD4⁺/CD8⁺in peripheralblood significantly increased after CIK cells transfusion, while such change didn’t happenin the peripheral blood of patients who received chemotherapy alone.③The medianoverall survival time of chemotherapy combined with CIK group and chemotherapy alonewere66months（95%CI:47.04⁸5.09months） and45months（95%CI:）arespectively, thedifference had no statistical difference （χ²=1.710，P=0.191）. The median progress-freesurvival time of the two groups were33months （95%CI:18.830⁴8.570months） and17 months（95%CI:8.441²6.22months） respectively, the difference was statisticallysignificant difference （χ²=6.768，P=0.009）.Conclusions Both the cell number and cytotoxic activity were highest on day14andthese CIK cells were suitable for transfusion into patient. In patients of malignantmelanoma with surgical resection CIK transfusion can improve their immunologic functionafter chemotherapy and prolong the progress-free survival time.