The Role Of Hepcidin In Iron Accumulation In Dopamine Nerve Cells In Vitro

AbstractObjective To study the activation of hepcidin (a iron-regulatory hormone) on intracellular iron regulatory.Methods After treatment with100μmol/L ferric ammonium citrate (FAC) for4h in vitro, hepcidin mRNA level were detected by reverse transcription-polymerase chain reaction and FPN1protein level were determined by western blotting. Then FPN1protein levels of pCMV-XL4-hepcidin transfected and hepcidin-treated cells were observed using western blotting analysis.Results Hepcidin mRNA level was up-regulated and FPN1protein level was down-regulated in MES23.5dopaminergic cells in a period of4h incubation with iron (P<0.05). Both in pCMV-XL4-hepcidin transfected and hepcidin-treated cells, low FPN1protein levels were observed(P<0.05).Conclusion The role for intracellular hepcidin generated in the dopaminergic cells is particularly relevant to restrict iron release by down-regulation FPN1expression in this region,thus an important contributor to the abnormal iron deposit occurred at an early stage in conditions of peripheral iron intoxication.