Altered Perlecan Expression In The Formation Of Epidural Scar After Posterior Lumbar Laminectomy

Objectives To make a research on the effect of perlecan in the formation of epidural scar after posterior lumbar laminectomy.Methods60Wistar rats were randomly and evenly divided into five groups:the experiment groups including A (the first week after surgery), B (the second week), C (the fourth week), D (the sixth week) and the control group. Laminectomy was performed on lumbar vertebra in each experiment. Then hematoxylin-eosin staining (HE) was done to observe the formation of epidural scar. Immunohistochemistry was performed to detect the perlecan located in the epidural scar. Western Blot and RT-PCR were made to quantify the expression of the perlecan after surgery.Results According to HE, with prolonged, the scar tissue gradually formed. Immunohistochemistry demonstrated that the perlecan was expressed in the fibroblast. Western blot and RT-PCR showed the high level expression of perlecan in the experiment groups compared to the control group (P<0.01). Furthermore, the expression of perlecan gradually increased with the prolongation of exposure time and had significantly statistical significance (P<0.01) between the first three experiment groups. However, the expression of perlecan in the sixth week decreased obviously compared to the fourth week group (P<0.01).Conclusion Perlecan was expressed in the fibroblast of the epidural scar. The perlecan plays an important role in the formation of epidural scar.