The Expression Of Interleukin-1β Of Epithelium In Aspergillus Fumigatus Keratitis In Rats And Its Relationship With Dectin-1

Objective:To observe the expression of interleukin-1p of epithelium in Aspergillus fumigatus keratitis in rats and its relationship with dectin-1. Materials and methods:90Wistar rats were randomly divided into5groups. Group A was blank control group (6rats). Group B (30rats) was Aspergillus fumigatus keratitis group. Group D (12rats) was Aspergillus fumigatus keratitis group treated with dectin-1antibody. After the epithelium of central cornea were scraped, Aspergillus fumigatus hyphae-laden contact lens were covered in the corneas of right eye, and the surgical control eyes were covered with PBS contact lens. Epithelium of corneas were collected at4h,8h,16h,24h in Group B、 C and24h in Group D、 E after infection. The expression of dectin-1and IL-1β in epithelium was detected by inmmunohistochemistry and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR).Results:The expression of IL-1β in epithelium of corneas was a little in the blank control group and the control group. After infection, the level of IL-1β increased with the time-lapse. The expression increased rapidly before16h, and then a little slower. The expression of IL-1βshowed significant difference at the observing points (P<0.01). The fundamental expression of dectin-1was detected in epithelium of corneas in the blank control group and the control group. After infection, the level of dectin-1increased (P<0.01). Blocking dectin-1with antibody can significantly inhibit the expression of IL-1β in epithelium (P<0.01).Conclusion:1. The successful fungal keratitis mode established by HE staining, isolation and cultivation of the Aspergillus fumigatus.2. IL-1β was detected in the epithelium of Aspergillus fumigatus keratitis in rats. IL-1β participates in the occurrence and development of fungal keratitis and it is a sensitive factor in fungal keratitis.3. After infection, the expression of dectin-1increased. Blocking dectin-1with antibody can inhibit the expression of IL-1β. Dectin-1may play an important role in the recognition and reaction of corneal epithelium to Aspergillus fumigatus.