Relationship Between ANTI-P53in Serum And The Familial Clustering Of Hepatocellular Carcinoma In Guangxi

Objective:To assess the correlation between the familial clustering of hepatocellular carcinoma (HCC) and the level of anti-P53in human serum in GuangXiMethods:With the same sexuality, age±5years, same ethnic group, same living environment and same babits as paried condition, take members of families with clustering of HCC from Guangxi Zhuang and Yao population as the experimental group, members without any cancer families as the control group, a total of164pairs object was involved in research, and HBV and HCV infection serological markers were measured as a confounding fator Enzyme-linked immunosorbent assay (ELISA) was used to detect the anti-P53in164members from20HCC clustering families and164memmbers from control families without any cancer. Univariate analysis was performed to assess the correlation between the serai level of P53antibody and the familial clustering of hepatocellular carcinoma in Guangxi. As well as comparative analysis serum P53antibody lever between relatives of different degree from families with clustering of HCC.Results:(1) The level of P53antibody was significantly higher in the members of families with clustering of HCC than that of the control families (Z=-3.04, P=0.002).(2) After eliminating the interference of hepatitis B virous infection, The level of anti-P53expression still higher in the members of families with clustering of HCC than that of the control families (P=0.011).(3) And there was significantly differences between relatives of different degree from families with clustering of HCC(x2=11.593, P=0.021), with the expression of anti-P53declining along with the decrease in relationship coefficient.(4) Furthemore,the high rate of the level of anti-P53expression was signifcantly greater in the members of families with clustering of HCC (95/164) compared to those of control families (71/164)(P=0.006).(5) and which was rising along with the increasing of the numbers of HCC in the members of families with clustering of HCC (x2=16.068, P=0.000).(6) Anti-P53level was also greater in HCC families featuring sibling affection than parental affection which attack compatriot than those heredity from parents(x2=12.679P=0.002).(7) The Yao population’s anti-p53was lower than the control (Z=-2.322,P=0.02).(8) Univariate analysis indicated that high expression of anti-P53is a risk factor for clustering of HCC families (OR=2.087,95%Cl:1.270-3.431)Conclusion:(1)Anti-P53may be related to clustering of HCC familes and high leve of anti-P53expression may be a factor for clustering of HCC familes in Guangxi, China.(2) The members of families with clustering of HCC may carry a genetic mutant lead to the familial aggregation of HCC.(3) Environmental combine genetic variation may contribute to the high leve of anti-P53expression,environment may play a role in the genetic model as a motivating fator.(4) There may be different state of inactivation of P53in Guangxi HCC high incidence areas.