The Effects Of Pas-na On Amino Neurotransmitters And Iron Metabolism Of Thalamus And Basal Nuclear In Mn-exposed Rats

Objective To study the effects of sodium para-aminosalicylic acid (PAS-Na) on the impairs of amino neurotransmitters and the iron metabolism disorder of thalamus and basal nuclear in manganese-exposed rats. Methods130male SD rats were divided into five groups randomly:include Mn-exposed (Mn), PAS-Na-prevention (P-PAS), low-dose PAS-Na treatment (L-PAS), mid-dose PAS-Na treatment (M-PAS), high-dose PAS-Na treatment (H-PAS) and control. The observation periods respectively for4weeks (Mn-exposed for4weeks),8weeks (Mn-exposed and PAS-Na prevented at the same time for8weeks),12weeks (Mn-exposed and PAS-Na prevented at the same time for12weeks) and18weeks (after Mn-exposed for12weeks then PAS-Na treated for6weeks). Administrate the groups of Mn, P-PAS, L-PAS, M-PAS, H-PAS intraperitoneal injection (ip) of MnCl2·4H2O15mg/kg, control group receive ip parallel volume NS, once per day, 5days/week, for4,8, or12weeks. And P-PAS group wnen receive ip MnCl2·4H2O15mg/kg, at the same time receive back subcutaneous injection (sc) PAS-Na200mg/kg, onec per day,3days/week, for8or12weeks. Then administrate the groups of L-, M-and H-PAS of back sc PAS-Na100,200or300mg/kg, the Mn and control groups receive sc parallel volume NS, onec per day,7days/week, for6weeks. Measure rats body weight weekly. Use high performance liquid chromatographic(HPLC) fluorescent assay estimate the concentrations of glutamic(Glu), glutamine (Gln), glycine(Gly), y-aminobutyric(GABA)in thalamus. Use rat’s brain tissue ferroprotein and transferrin ELISA kit test the concentrations of ferroprotein and transferrin in thalamus and basal nuclear. Results In The observation periods for4,8, and12weeks, the body weight decreased in Mn group rats compare with control from the first week, and increased in P-PAS group compare with Mn in observation periods for8and12weeks. In The observation periods for18weeks, after cease Mn-exposed, the body weight of Mn group rats restore, and also in the different doses PAS-Na treated groups, the weight growth rates increased and close to the control group. In observation periods for4,8, and12weeks, there was no significant in the thalamus concentrations of Glu, Gln, Gly, GAB A. In observation periods for18weeks, the Mn group Glu level decreased compare with control, and compare with Mn group the Glu level increased in L-PAS group(P<0.05). In thalamus, the alteration of ferroprotein and transferrin concentrations was no significant in the observation periods for4,8and12weeks, but in the observation periods for18weeks, the transferrin level in Mn group higher than the control(P<0.05), and decreased by PAS-Na treatment(P<0.05), in the H-PAS decreased most(P<0.01). And in basal nuclear, there was no significant of the change of ferroprotein and transferrin concentrations, but in the observation periods for18weeks, the transferrin level in L-PAS group lower than the Mn group significantly((P<0.05)). Conclusions (1) Mn-exposed can reduced the rats body weight growth rates;(2) the impair of Glu and Gln in thalamus by Mn-exposed is persistent, PAS-Na may have antergic in the reduced of Glu concentration in thalamus; and (3) PAS-Na treated may decreased the transferrin level of Mn-exposed rats in thalamus and basal nuclear.