Study On Extraction And Separation Technology Of Procyanidins From Lotus Seed Pot

Lotus Seed Pot belongs to mature torus of Nelumbonaceae medicinal plant, which contains rich procyanidins and is good Chinese herbal medicine. A lot of them abandoned in processing process of Lotus Seed Pot, and resources are not make full use of. Extracting procyanidins from Lotus Seed Pot actually belongs to make use of waste. Research and development procyanidins of Lotus Seed Pot not only have scientific value but also bring huge economic benefits. Therefore, this paper chooses Lotus Seed Pot as the source of the procyanidin extraction. In order to provide the basis of procyanidins from Lotus Seed Pot for industrialization production, extraction and purification technology of procyanidins from Lotus Seed Pot were studied.The following are the several aspects of work.1. The content of procyanidins in the Lotus Seed Pot was determined by vanillin-hydrochloric acid assay methodology. The optimum reaction condition was obtained through studying the factors affecting the colorimetric reaction including hydrochloric-acid acidity, vanillin concentration, reaction temperature and reaction time, etc. The optimum conditions of analysis were:vanillin concentration20g/L (methanol), hydrochloric acid acidity100%, reaction temperature20(±1)℃, reaction time20min. Under the above conditions, the linearity range was40～200μg/mL, equation of linear regression was y=0.00424x-0.02286, regression coefficient was0.99997, lowest detection limit was4.0μg/ml.2. On the basis of single-factor test, the response surface methodology(RSM) with4factors and3levels was adopted to optimize ethanol extraction technology of procyanidins from Lotus Seed Pot (LSPC). The interactive effects of the parameters on LSPC yield were investigated. Design Expert7.1.6software was used to fit experimental data and a quadratic polynomial regression model was developed for LSPC yield as a response to the parameters. Results showed that the optimal value of extraction conditions were as follows:ethanol concentration50%, solvent/material ratio25mL/g, extraction temperature55℃and extraction time60min.Under such conditions and extraction1time, the yield of LSPC was up to6.678mg/100g, which closed to the predictive yield(6.693mg/100g). This model is reasonable and reliable, and can be used in the actual forecast.3. The macroporous resin adsorption method was adopted to separate procyanidins of Lotus Seed Pot. Through the static adsorption and desorption tests and the adsorption dynamics tests,10types of macroporous resins were tested to separate procyanidins.The AB-8macroporous resin was selected as the best suitable resin. The suitable process conditions were determined through dynamic adsorption and desorption tests, the results showed that:300mL as the sample volume,2.0BV/h as adsorptive flow rate,100mL50%ethanol as the elution,1.0BV/h as desorptive flow rate. Under these conditions, AB-8macroporous resin was used to separate procyanidins of Lotus Seed Pot. The product yield closed to90.67%.The concentration of procyanidins was froml.809mg/mL up to4.236mg/mL.