Effect Of Endotoxin On ERK1/2Pathway Of Primary HSCs In Vitro

【Objective】To investigate the influence of endotoxin on ERK1/2pathway of primary HSCs invitro.【Methods】HSCs were isolated from rats liver by the method of pronase E and IV collagenasedigestion and density gradient centrifugation. The cell viability was determined by Trypan blueexclusion staining. The purity of HSC was identified by the expression of desmin usingimmunocytochemistry SASC method. After3days normal cultivation, HSCs were randomizedinto endotoxin group, PDTC(Pytrolidine-1-dithiocarboxylic acid, inhibitor of NF- B) groupand control group. Endotoxin and PDTC groups add endotoxin (lμg/ml), PDTC group addPDTC(15μmol/L) as well, the levels of P-ERK1/2were measured by western blot afterincubation for30minutes.【Results】The level of P-ERK1/2increased significantly after HSCs were stimulated directly byendotoxin, compared with the control group（P<0.01)，incubated with PDTC can partly preventthis change(P<0.05).【Conclusions】Endotoxin can directly stimulate ERK signaling pathway of HSCs, and NF-Bsignaling pathway may play an important role in this process.