The Influence Of Sirt1 On The Neurological Recovery After Spinal Cord Injury In Rats

Objective: To explore the relationship among SIRT1 and inflammatory cytokines,apoptosis,neurological recovery after spinal cord injury in rats.Methods: The SCI of animal model was made by using a modified Allen’s method onT(10).One hundred twelve SD rats were randomly divided into four groups：SHAMgroup(n=16),SCI group(n=32), RES group(n=32),SOL group(n=32).Resveratrol(100mg/kg)and Solutol(100mg/kg) was gave respectively by intraperitoneal injection following SCI.The expresstion of SIRT1 were measured by reverse transcription-polymerase chainreaction (RT-PCR). we detected the inflammatory cytokines ( IL-1β，IL-6，IL-10，TNF-α)and apoptosis with ELISA,TUNNEL respectively. The BBB scores before surgery and at1d,3d,1 week,2 weeks,3 weeks and 4 weeks after the surgery were examined.Results：All primers were amplified the target slices successfully.We have found that RESgroup ,SCI group and SOL group all raise up.But there was a remarkable increase in theSIRT1 level at 4 hours after the trauma and it got to the peak at 8h in RES group.Comparingto the other two groups(SCI group, SOL group), the expression of SIRT1 had significantlystatistical differences(p＜0.05) at 4h,24h,36h of RES group.Except the SHAM group,theSIRT1 expression level all descended after 8h.The expression of inflammatory cytokines increased obviously at 4h after trauma in SCIgroup,and the IL-6 got to the peak at 8h as well as TNF-α.While the peak of IL-1βlevelwas at 24h after trauma.But the level of IL-10 increased by degrees.The change rule ofinflammation cytokines in SOL group was agreed with the SCI group.The level of everyinflammatory cytokine in RES group has statistical differences compared with the other twogroups(p＜0.01).Compared with SHAM group，the number of cell apoptosis increased remarkably in SCIgroup，RES group，SOL group after one day injury,it had significantly statisticaldifferences(p＜0.05).and the number of cell apoptosis reached up to 54.82，38.49，53.24in SCI group,RES group，SOL group respectively.Compared with the other groups, the levelof RES group was lower,there were significantly statistical differences(p＜0.05).There was no remarkable change in sham group ,while the function of hind limbs in otherthree groups was loss completely after one day spinal cord injury.Although there were anelevator tendency from 3th to 21th in SCI group,SOL group and RES group,there were no statistical significance（p＞0.05）.At 28th,compared with SCI group and SOL group,theBBB scores in RES group got heighten obviously and had statistical significance（P＜0.05）.it is thus evident that SIRT1 promote the functional rehabilation after spinal cord injury inrats.Conclusions：SIRT1 can remarkably promote the functional rehabilation after spinal cordinjury in rats,by the mechanism of anti-inflammation and anti-apoptosis.The detailedmechanism needs further study.