The Effects Of Propofol Postconditioning On Expressions Of Toll-like Receptor4and NF-κB And Serum TNF-α In Hepatocytes After Ischemia Reperfusion Injury In Rats

Objective Research propofol on liver ischemia reperfusion rat liver tissue toll like receptor4(TLR4) and nuclear factor-JB (NF-κB) and serum tumor necrosis factor-a (TNF-a), and investigate the effects of propofol postconditioning acute liver injury induced by ischemia reperfusion in rats and possible mechanisms.Methods Sixty healthy Wistar rats not discriminated female and male, weighting200-250g were randomly divided into five groups(n=12).(1)sham operation group(S group):only laparotomy without clamping the hepatic portal;(2)ischemia-reperfusion group(IR group): restore perfusion after clamping the hepatic portal ischemia45min;(3)postconditioning group with lmg/kg propofol (group P1);(4)postconditioning group with2mg/kg propofol(group P2);(5)postconditioning group with4mg/kg propofol(group P4).A rat model of hepatic ischemia-reperfusion was established, after45mins reperfusion.P1,P2,P4groups were using physiological saline to propofol diluted to2ml, in five minutes before reperfusion with electronic trace pump via the femoral vein respectively uniform pumpingl,2,4mg/kg propofol. Group S and group IR infusion of an equal volume of saline solution. Each time all infusion5minutes. The animals were killed at3h of reperfusion respectively. Morphologic changes of the livers were examined, expression of Toll-like receptor4mRNA and NF-κB mRNA in liver tissues was assessed by Reverse transcription-Polymerase chain reaction, concentration of TNF-a in the serum was determined using radio-immunifaction method.Results1. The result of Light microscopy:Compared with S group,IR group showed great changes. The pathological lesions were lighter in P1group,P2group and P4group than in IR group.2. The results of every index:The expression of TLR4mRNA and NF-κB mRNA and serum TNF-a concentration were significantly higher in IR group,Pl group,P2group and P4group than in S group,the difference between groups has statistical significance (P<0.01). The expression of TLR4mRNA and NF-κB mRNA and serum TNF-a concentration were significantly lower in P1group,P2group and P4group than in IR group(P<0.01).Compared with P4group,the expression of TLR4mRNA and NF-κB mRNA and serum TNF-a concentration in P1group and in P2group were lower(P<0.01).Compared with P2group,the expression of TLR4mRNA and NF-κB mRNA and serum TNF-a concentration in P1group were higher.(P<0.01). Conclusion1.TLR4,NF-KB and TNF-a involved in hepatic ischemia reperfusion injury in formation.2. Propofol postconditioning can inhibit TLR4and NF-κB in liver tissue expression, reduce the serum content of TNF-a, propofol postconditioning on ischemia reperfusion induced liver injury maybe has a protective effect.