| Model Materials and methods: Select43Japanese big ear white rabbitsto establish the osteotomy and Bone extended model. After three eliminated,the remaining40randomized controlled trials design principles are divided intoA, B, C, D four groups. A group without any disposition as the blank controlgroup; Group B, in the beginning of the extension, simply extend the District ofpercutaneous injection of BMP-2; Group C, simply extracorporeal shock wavetreatment. Group D do ESWT+BMP-2joint intervention. Animals after theend of the bone lengthening respectively extend after the end of the day,14days,28days tibial lateral radiographs for the observation of bone lengtheningin shape, density and trabecular bone changes, the defect density changes.Respectively, extend the end of the first14days,28days in each group wererandomly selected two animals, after the death of air embolism, take the righttibia specimens, measurement of tibial lengthening in (C) and osteotomy of theproximal (P) and distal (D), bone mineral density values, the results of theformula (C/P+C/D)×0.5×100%the BMD,statistical analysis of all data x±s said, setting P <0.05was considered statistically significant. Take the tibiato take the extension of the bone specimens, and to make a0.5cm×0.5cm×0.5cm size, fixed, decalcified, embedded in paraffin, sliced5-6μm, HE stainingafter the light microscope.28days, group A shows a lot of woven bone filling,but still visible in a small amount of fibrous tissue, cortical bone formation isnot very obvious. Group C is basically woven bone filling the visible part of thecortical bone.Result: X-ray observation of bone extended by14days after the end,Group D bone extension of the District of callus formation was significantly more than other groups, groups B, C, no significant difference. Extended afterthe end of28days, Group B, prolong District callus more than group A, groupC also seen continuous callus, callus volume between B, and A, Group D calluswas significantly more than the other three groups,canal began to be formed;Histology by light microscopy observation of rabbit tibia after the end of14days, group A, group C only seen a small amount of osteoblasts, group B,fibrous tissue filling, osteoblasts than in group A and C increased significantly,showing a small amount of woven bone, group D reduced fibrous tissue, wovenbone increased significantly; Group B newborn cortical bone volume increasedsignificantly, then woven bone to reduce the medullary cavity formation is notobvious; Group D can be seen cortical bone is continuous, but a small part ofthe bone defect, the formation of part of the canal; Bone mineral densityanalysis draw day14with the control group A to group B, group D werestatistically significant (P <0.05), Group C no statistical significance; Day28compared with the control group A three group B, group C, D group werestatistically significant, Group D and group B and group C were statisticallysignificant.Conclusion: Bone lengthening in rabbit tibial lengthening in the localinjection of BMP-2to promote bone regeneration has a positive effect; ESWTto stimulate bone regeneration in rabbit tibia lengthening in with good effect,but the apparent role of slower BMP-2. The two factors co-additive effect incombination is better able to stimulate bone lengthening boneless callusformation. |
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