Treatment Of Rats With Acute Cerebral Ischemia By Optimized Novel Human Immunodeficiency Virus Type-1(HIV-1) Lentiviral Vector Containing Vascular Endothelial Growth Factor164and Enhanced Green Fluorescent Protein

growth factor164and enhanced green fluorescent proteinCerebral infarction is the most common diseasee in nerve system which has highmorbidity、high disability and high mortality rate. It threats human health and quality of life.Objective: Inject optimized novel human immunodeficiency virus type-1（HIV-1）lentiviral vector containing vascular endothelial growth factor164and enhanced greenfluorescent protein into animals with transient focal cerebral ischemia-reperfusion model andexplore the role of VEGF in acute focal cerebral ischemia disease.Materials and Methods: Transient focal cerebral ischemia-reperfusion of left middlecerebral artery occlusion in rats was established by suture occlusion method. One thousandmale Wistar rats weighing250to300g were used. The animals were randomly divided intofive groups: normal group8(group A), sham group8(group B), ischemic group24(group C),PBS-treated group24(group D), VEGF-treated group36(group E). All groups were dividedinto four points1d,3d,7d and14d group according to different time after infarctiondetection.①The neurological behavior scores of operation and treated group were evaluated postoperation, and the rat with1-3score was selected into experimental model.②Brains were taken according to different time. Cerebral infarction was observed byTTC staining.③Observe the histopathologic change in brain by means of HE.④Measure the expression of VEGF、CD34and CD133in ischemia penumbra bymeans of immunohistochemistry. The positive cells were observed through microscope. Theresults were analyzed by SPSS18.0software.⑤Detect EGFP by immunofluorescence stain.Result:①Transient focal cerebral ischemia-reperfusion of left middle cerebral artery occlusionin rats was established by suture occlusion method.②The neurological scores were lower in group E than other groups, especially after7 days of treatment.③TTC staing: There was no significant differences between C and D group. Totalinfarct volume were significantly reduced after temporary MCAO in group E compared togroup C and D（P<0.05）.④Expression of VEGF、CD34and CD133: The expression of VEGF and CD34increased significantly in group E than other groups. CD133was not expressed in eachgroup.⑤Immunofluorescence staining showed: there was green fluorescence in VEGF-treatedrats compared to other groups.Conclusion:①Treatment of acute ischemic stroke with optimized novel human immunodeficiencyvirus type-1（HIV-1） containing vascular endothelial growth factor164and enhanced greenfluorescent protein has good effect, can promote angiogenesis, save ischemic penumbra andimprove the recovery of injured nerve.②Optimized novel human immunodeficiency virus type-1（HIV-1）containing vascularendothelial growth factor164and enhanced green fluorescent protein can decrease infarctionvolume after acute ischemic stroke. Alleviate the edema of nerve cells and interstitial,improves neurological deficits.