The Study Of The Relativity Between ATX And The Invasion Of Glioma

Objective: The experiment indicated the function that ATX made inpectin tumor invading behavior.Method: The experiment used human brain pectin tumor U251、U87MGduring logarithm growth period as targets, then assorted them to blankgroup（DMEM culture medium）、L-His group（0.01mol/L）、L-Histidine+Zn²⁺group(including0.01mol/L L-His and0.25mmol/L Zn²⁺).Under their owncondition, The experiment mensurated albumen contents of ATX、FN、LPAR1、LN that existing in these groups with ELISA method, and alsomensurated these group cell’s invading capabilities with Transwell zeta method.Results: ELISA method detected albumen contents of ATX、FN、LPAR1、LN that existing in U251、U87MG cells after48hours incubating.Result indicated that ATX、FN、LPAR1、LN had shown in all the groups cells;the albumen contents of ATX、LPAR1in L-His cells were nearly equal to thecomparison group and L-His+Zn²⁺cells（P＞0.05）; the albumen contents ofLN、FN in L-His cells were higher than the comparison group and L-His+Zn²⁺cells（P＜0.05）. The experiment result indicated that all groups pectin tumorwere invadable, and L-His cells had the lower invasiveness than thecomparison group and L-His+Zn²⁺cells（P＜0.05） when penetrating Matrigelartificial fundus velum.Conclusion:1、ATX could reduce the level of LN、FN, then acceleratedpectin tumor’s invading, with no affection to LPAR1.2、L-His cells exerted influence by restraining ATX, but had no obviouseffect on ATX.