Distribution Diversity Of Integrins And Calcium Channels On Major Human And Mouse Host Cells Of Leptospira Species

Objective:To determine the distribution and its diversity of integrins and calcium channels on major human and mouse host cells of Leptospira species.Methods:Using immunofluorescence assay, the expression of β1,β2and β3integrins on the surface of human monocyte line THP-1, mouse mononuclear-macrophage-like cell line J774A.1, human vascular endothelial cell line HUVEC, mouse vascular endothelial cell EOMA, human hepatocyte line L-02, mouse hepatocyte line Hepal-6, human renal tubular epithelial cell line HEK-293, mouse glomerular membrane epithelial cell line SV40-MES13, mouse collagenoblast line NIH/3T3, human and mouse platelets was detected. On the other hand, the distribution of voltage gate control calcium channels Cav3.1, Cav3.2, Cav3.3and Cav2.3, and receptor gate calcium channels P2X1, P2X2, P2X3, P2X4, P2X5, P2X6and P2X7was also determined using Western Blot assay.Results:β1integrin proteins express on the membrane surface of J774A.1, THP-1, HUVEC, EOMA, L-02, Hepal-6and HEK-239cells as well as human and mouse platelets. β2integrin proteins express on the membrane surface of J774A.1, THP-1, HUVEC, EOMA, and NIH/3T3cells. β3integrin proteins express on the membrane surface of J774A.1, THP-1, HUVEC, EOMA, Hepal-6, HEK-239and NIH/3T3cells as well as human and mouse platelets. P2X1receptor gate calcium channel expresses on the membrane surface of human and mouse platelets, while P2X5receptor gate calcium channel expresses on the membrane surface of J774A.1, THP-1, L-02, Hepa1-6, HEK-239and HUVEC cells. However, the other calcium channels could not been found on any of the tested cells or platelets.Conclusion:There is a large distribution diversity of integrins and calcium channel proteins on the major human and mouse host cells of Leptospira species, which may be associated with the difference of leptospire-induced injury in different tissue cells.