Detection Of The47-kilodalton And The15-kilodalton Membrane Immunogen Gene Of Treponema Pallidum In Whole Blood Of Patients With Syphilis

Background:Sypilis, a kind of fectious disease which is caused by7reponema pallidum(TP),is systemic and chronic, and it can attack nearly all of the organs of people and has a long course of disease and complex clinical manifestations.what’s more.it can cause fetal congenital syphilis and adverse pregnancy through the placenta spread,then the health of the next generations are compromised by sypilis. The widely prevalence and dissemination of syphilis have become the public health problem of all countries in the world.as a resultthe normalized treatment of syphilis has been highly appreciated’1’.The principles of treatment of syphilis include well-defined diagnosis.early treatment,doses sufficient,treatment rules,the follow-up after treatment,the examination and treatment of sex partner or spouse,the prohibition of sexual intercourse during treatment,and striving to achieve the curation of clinical and serum[2].However.when the doctors diagnose syphilis under some circumstances,biologic falsepositive or falsenegative test for syphilis happenes,which poses great difficulty in the diagnosis and treatment of syphilis.The clinical immunology is the hot spot of all kinds of research about syphilis.Syphilis was confirmed the gene sequence with the continuous development of gene cloning technology for the past few years, and the outer membrane protein such asTpN15,TpN17,TpN44.5,TpN47and so on can be used for syphilis helicoid detection in the coding of many of the antigen[3.4]. There has not been a report about diagnosing syphilis by detecting the two membrane immunogen genes.that is TpN15and TpN47,both at home and abroad at present.The objective of this study is to eliminate serological false positive test for syphilis by detecting the two membrane immunogen genes.that is TpN15and TpN47,under the way of the nested polymerase chain reaction (PCR)assay.Objective:To investigate the contribution of a nested polymerase chain reaction (PCR)assay to eliminate serological false positive test for syphilis.Methods:Routine PCR and nested PCR assays were performed to amplify specific fragments of the47-kilodalton and the15-kilodalton membrane immunogen gene of treponema paliidum from90whole blood samples of persons with suspected syphilis. At the same time,we compared nested PCR assays with serological test.Results:By detecting the90tests performed, and directly comparing with serology, we found that nested PCR showed96.7%agreement, with a sensitivity of93.3%and a specificity of96.7%. There were significant differences between the two PCR assays in detection of TP from whole blood and the nested PCR was more sensitive than the routine PCR (P≤O.01).Conclusions:The nested PCR assay is more sensitive than the routine PCR assay in detecting low numbers of TP in whole blood samples which can eliminate serological false positive test for syphilis. so that we can improve the accuracy rate of diagnosis of syphilis.