| Background and Aims:Foxp3+Treg cell is a kind of immunoregulatory cells and plays a critical role in immune regulation and maintaining an immune balance. The aims of present study were to investigate the effects of Faecalibacterium prausnitzii (FP) on CD4+CD25+Foxp3+Treg cells in the peripheral blood and spleen and cytokines in rats with ulcerative colitis and to explore the mechanism of FP in the treatment of ulcerative colitis (UC).Methods:Total of60SD rats were randomly divided into six groups which were normal control group (A), colitis group (B), FP group(C), FP supernate group(D), bacteria culture medium group(E) and bifidobacterium group(F), respectively. The model of experimental colitis in rats was established by enema with2,4,6-trinitrobenzene sulphonic acid (TNBS). The score of macroscopic injury and histological change of colon were observed. The proportion of CD4+CD25+Foxp3+Treg cells in peripheral blood and spleen were detected by flow cytometry. Levels of IL-6, IL-10, IL-12and TGF-β1in plasma were detected by ELISA. The content of short chain fatty acids (SCFA) in the feces was detected by gas chromatography.Results:Comparing with colitis group (group B), the macroscopic injury of colon in FP group (group C), FP supernate (group D) and bifidobacterium (group F) was obvious improved. The proportion of CD4+CD25+Foxp3+Treg cells in the peripheral blood and spleen and the content of SCFA in group B were2.21±0.75%,3.15±0.45%, and33.52±9.5μmol/g, respectively, whic were signifiantly decreased compared to group A(3.24±0.66％,4.19±1.02％,43.194±9.5μmol/g)(P＜0.05).Afer the treatment of FP,FP supernate and bifidobacterium for7days,the proportion of CD4+CD25+Foxp3+Treg in the peripheral was2.97±0.44％,3.33±0.51％and2.64±0.91％,while in the spleen was3.87±0.92％,5.99±1.82％and3.27±1.51％,respectively,and the content of SCFA was39.63±14.51μmol/g,43.67±13.98μmol/g,36.93±8.4μmol/g, respectively. Comparing with group B,the levels of cells and SCFA in group C and D were noticeably increased(P＜0.05； P＜0.01).The contents of IL-6(111.48±10.34pg/ml)and IL一12(185.48±13.35pg/ml)in group B were increased while the IL-10(87.17±3.2pg/ml),TGF-β1(71.31±5-32pg/ml)and the ratio of IL-10/IL-12(0.47±0.21)were significantly decreased compared to goup A(49.41±5.79pg/ml,91.56±7.53pg/ml,94.31±5.25pg/ml,109.68±6.54pg/ml and1.03±0.20,respectively)(P＜0.05). Comparing with group B, the levels of IL-10in group C (107.71±5.90pg/ml), D (108.84±4.17pg/ml)and F(116.15±3.28pg/ml)were increased significantly(P＜0.05).The levels of IL-6(56.70±5.82pg/ml,51.19±6.47pg/ml,55.39±7.87pg/ml)and IL-12(100.66±9.01pg/ml,111.07±8.70pg/ml,109.58±7.90pg/ml)in group of C,D and F were significantly lower than that in groug B(P＜0.05),and the ratio of IL-10/IL-12(1.07±0.67,0.98±0.34,1.06±0.38)and TGF-β1(302.02±2.23pg/ml,270.17±2.32pg/ml,118.09±3.97pg/ml)were increased significantly(P＜0.05). Comparing with group D,the proportion of CD4+CD25+Foxp3+Treg cells in the peripheral blood and spleen(2.15±0.3％,3.70±0.73％)and the content of SCFA(27.02±11.02μmol/g)in group E were decreased significantly respectively(P＜0.05).The levels of IL-10(84.24±3.00pg/ml), TGF-β1(108.65±3.00pg/ml)and ratio of IL-10/IL-12(0.47±0.29)were decreased significantly (p＜0.05),while the IL-6(108.27±8.14pg/ml)and IL-12(179.24±11.3lpg/ml)were increased significantly in group E compared to group D(P＜0.05).Conclusioms:The results indicate that FP and FP supernate have an obvious therapeutic effect on the TNBS-induced experimental colitis in rats.The mechanism may be related to increasing the proportion of CD4+CD25+FOXp3+Treg cells in peripheral blood and spleen,increasing TGF-β1and the ratio of IL-10/IL一12and decreasing IL-6. |
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