Anatomy And Main Chemical Components Of Hydrocotyle In Sichuan

Objective:Based on the plant taxonomy and germ plasm resource of Hydrocotyle, study on the anatomy and main chemical components to searching genetic relationship in Hydrocotyle, use Centella asiatica as control sample to investigate and analysis that traditional efficacy association with the chemical components in5plants of Hydrocotyle. Laying the foundation for the quality specification formulation and quality control and providing a reliable basis for clinical medicine and further study on the pharmacodynamics of Hydrocotyle.Method:Content retrieval a book on Chinese herbal medicine and literature to find out historical background of Herba Hydrocotylist and correlation between traditional efficacy and chemical components; Using the knowledge and experimental technique of botany, plant anatomy and identification of Chinese pharmacy for the taxonomic treatment and identification of Hydrocotyle; Use UV, TLC instrumental analysis and pharmaceutical analysis, to screen the representing components for traditional efficacy in different idioplasm of Hydrocotyle. To analyze the difference in different idioplasm by HPLC while the triterpenoid and flavone were determined in different idioplasm and different medicinal part of Herba Hydrocotylist and the chromatographic conditions was optimized by HPLC.Result:There are6species include1variant of Hydrocotyle in Sichuan province and5species include1variant officinal. The pennywort infructescence is different from another Herba Hydrocotylist, it valuable to further study. H. wilfordi and H. nepalensis have two germ plasm red and green in the natural, and the flavone content in red are higher than green. H. sibthorpioides is similar to H. sibthorpioides var. batrachium in microstructure and cell structure; H. nepalensis, H. podantha and H. wilfordi are much alike in microstructure. There are all5medicinal materials has contains asiaticoside, madecassoside, quercetin, kaempferol. The triterpenoid content of herba hydrocotylist are higher than Centella asiatica and the flavone content lower than Centella asiatica.Conclusion:On the base of herbs character and microstructure can divide Herba Hydrocotylist into two groups:H. sibthorpioides and H. sibthorpioides var. batrachium are leaflet seta group, H. nepalensis, H. podantha and H. wilfordi are leafy columnar glandular group. The asiaticoside and madecassoside content of leafy group is higher than leaflet group, but the flavone content of leafy group is lower than leaflet group. The madecassoside content of H. nepalensis is reached0.8%, so the plant of H. nepalensis can use to extract madecassoside. The characteristic variable of anatomy and classification of herba Hydrocotyle plants give maintenance logistics. The method of TLC and HPLC for determination was well stability and reproducibility, providing reliable basis for quality control in medicinal materials and its preparation. The traditional efficacy was closly related to the modern pharmacological action on asiaticoside, madecassoside, quercetin and kaempferol. And this results laying the foundation for study of the Chinese medicine theory with target theory in modern pharmacology.