| Objective: To investigate the effect of exogenous hydrogen sulfide （H2S） on ferricchloride （FeCl3）-induced thrombosis in SD rat carotid and examine the possiblemechanisms involved.Methods: Male SD rats, weight320-400g, were used in this stduy. In brief, the ratcarotid artery was dissected from other tissues.10%FeCl3solution was then appliedonto the carotid artery for5min to induce arterial thrombosis. the blood flow velocitywas monitored by laser Doppler flowmeter. Both wet weight and dry weight ofthrombus were recorded. Histomorphological study was used to observe the effect ofexogenous H2S on vascular endothelial injury induced by10%FeCl3solutions. MaleKunming mice,18-25g, were also included in this study. Filter papers were used tomeasure tail vein bleeding time; Slide method was applied to measure mice clottingtime.Results: In the present study, we find that：（1） Rat carotid artery thrombosis wasmarkedly reduced by different doses of sodium hydrosulfide solution;（2）Thehematoxylin eosin staining sections show that hydrogen sulfide may protect the carotidartery endothelial cells to reduce ferric chloride-induced thrombus formation.（3） LaserDoppler flowmeter study showed that hydrogen sulfide could alleviate the decreaseof blood flow rate due to thrombus formation induced by10%FeCl3. The data indicatethat H2S could attenuate thrombosis;（4） The mice tail vein clotting time and bleedingtime experimental studies showed that sodium hydrosulfide solution can significantlyprolong the bleeding time （BT） and clotting time （CT）, suggestingthat exogenoushydrogen sulfide may reduce thrombus formation through regulating platelet function.Conclusion: Exogenous hydrogen sulfide could reduce the ferric chloride-inducedthrombosis formation in rat carotid artery. Exogenous hydrogen sulfide may exertprotective effect against ferric chloride-induced endothelial injury. This gasotransmitter could also prolong the clotting and tail bleeding time in mice. |
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