The Prepared And Characterization Of Hyaluronic Acid Micro-nano Structures And Their Effects On Cell/Bacteria In Vitro

Implanting cardiovascular materials must be provided with favorable hemocompatibility. Endothelialization on the surface of the implanting materials is always one of the ideal method to improve the hemocompatibility. In this thesis, capillary force lithography is used to fabricate the hyaluronic acid (HA) micropattern on the activated titanium (Ti-OH) surface. The HA micropatterns were characterized by scanning electron microscope(SEM), light microscopy, fourier transform infrared spectroscopy(FTIR), contact angle measurement, surface profiler. Surface profiler and light microscopy results showed that polydimethyl-siloxane (PDMS) stamp with high fidelity. The results of SEM, FTIR and contact angle measurement showed that the morphology of Ti surface activated by NaOH solution were changed, its roughness and hydrophilicity increased and ophilic groups was detected on the surface. Light microscopy and surface profiler results demonstrated that HA micropatterns had been prepared accurately on the surface of Ti-OH. Co-culture of human umbilical vein endothelial cells with human umbilical artery smooth muscle cells on the HA micropattern. The morphology and distribution of the co-culture Ecs is more similar to that in vivo.Biomaterial centered infections remain the major and often irreducible complication in the clinical use of biomaterials. To date, many kinds of antibacterial biomaterials have been studied for the prevention or treatment of bacterial infection on implanted devices. The nano-silver is a safe, highly effective new antimicrobial agents. Nano-silver is applied to the hunman implants to make sure that the implanted devices themselves possess antimicrobial function. HA was used as the stabilizing agents and the templates to prepare metal nanoparticles by photo-reduction of silver nitrate. The properties of the silver nanoparticles were investigated by UV-Vis spectroscopy, scanning electron microscope(SEM), Energy Dispersive X-Ray Spectroscopy (EDX), dynamic light scattering (DLS), Zeta potential instrument, antim icrobial activity test and cell culture experiments. Results show that the obtained silver nanoparticles are spherical and the average diameter is about170nm. Furthermore, the silver nanoparticles exhibit antibacter ialproperties against Escherichi a coli. However, the cytotoxieity of silver nanoparticles is to be studied further more.