| Raman spectroscopy is widely used in many fields of science and allows one to derive detailed and specific information on a molecular vibration and rotation. By using surface-enhanced Raman technology, material detection sensitivity are greatly improved and it gradually as a precise technique for qualitative and quantitative analysis of the material.This paper introduces the basic theory of Raman spectroscopy and elaborate the application and study progress of surface-enhanced Raman spectroscopy. With the finite difference time domain method for enhancing substances, in theory, simulation, and finally explore the use of surface enhanced Raman spectroscopy method of quantitative detection of thyroid stimulating hormone concentration in filter paper blood spots.Preliminary study on the quantitative determination of thyroid stimulating hormone (TSH) in newborn blood by Surface-Enhanced Raman Spectroscopy(SERSp)-Partial Least Squares Regression (PLSR)based approach. The date of TSH concentration which obtained by SERSp-PLSR compared with the date form time-resolved fluoroimmunoassay to validate the feasible of the SERSp-PLSR method. An improved polynomial fitting method was used for baseline correction, which could remove the error of result caused by the fluorescence and baseline drift, the intensity of725cm-1Raman peak was used as a normalization standard, the ratio of the intensity of other Raman peak with the intensity of725cm-1Raman peak were used for measuring its concentration. And use of partial least squares regression fitting regression equation, which was used to obtain the other unknown samples of TSH concentration. This experiment is the first use of SERSp for quantitative detection of TSH concentration in the blood, the experimental results consistent with the actual but still need further improvement. To some extent, this method is feasible, and also develop a quick and easy path for the quantitative detection of TSH concentration. |
