The Pharmacokinetic Impact Of Oral Tripterygium Wilfordii Hypaconitine By Electroacupuncture In Rheumatoid Arthritis Rat Models

Objective:The effect of electroacupuncture combined with triptolide on the efficacy of rheumatoid arthritis in joint swelling in rats, and acupuncture to rat models of oral tripterygium wilfordii hypaconitine, pharmacokinetic of the main parameters of the effects to provide experimental basis for clinical acupuncture combined with the treatment of rheumatoid arthritis.Methods:(1) using complete Freund’s adjuvant (CFA) will cause rheumatoids arthritis rat model;(2) using liquid chromatography-mass spectrometry (LC-MS-MS) in Thermo BDS HYPERSIL C18(100x2.1mm,2.4u m) for stationary phase chromatographic column to55of ammonium acetate acid and volume and45a volume methanol as mobile phase, flow rate:0.2ml/min as the main chromatographic conditions. With electrospray ion source (Turbo Spray); Positive ions mode as the main mass spectrometry conditions, on plasma samples for analysis. Medicine is consecrated to different time points after rats plasma, join internal standard prednisolone, using liquid-liquid extraction to join a certain volume of ethyl acetate, according to a certain proportion drain on the fluid. Clear liquid on the swing dry, the current phase solubility residue junior sample analysis, each time point measured drug concentration in the plasma, through the DAS2.0pharmacological software analysis pharmacokinetic parameters, and discusses the model of the drug in the body of the metabolic regularity and observe the acupuncture pharmacokinetic influence.Results:(1) the different methods of rheumatoid arthritis rats after intervention are both a swelling of the toes without the interference of the model rats have improved, specific improvement:acupuncture medicine combining group> simple medicine group> simple acupuncture group.(2) LC-MS-MS detection model rats plasma tripterygium wilfordii hypaconitine, concentration and peak area ratio good linear relationship, plasma tripterygium wilfordii hypaconitine, minimum detect low limit for0.25ng/ml. Plasma sample extraction recovery can be amounted to89.7%. Days and daytime differences RSD less than15%.(3) the acupuncture affected the rat models of oral tripterygium wilfordii hypaconitine in major pharmacokinetic parameters, improving the blood drug concentration.