Bioactive Metabolits From The Two Fungi Associated With The South China Sea Holothurian Holothuria Nobilis Selenka

Differing hugely in entironment makes the marine organism’s secondarymetabolite biosynthesis and response system huge difference of the terrestrial biological,,marine organisms show significant difference from terricolous organisms in secondarymetabolites concerning their chemistry and bioactivity, and are therefore regarded asirreplaceable resources in new drug discovery.In recent years, growing interest in marinenatural products has led to the discovery of an increasing number of bioactive secondarymetabolites from marine fungi and make it become a hot research area. And marine fungihave been a rich source of pharmaceutically important compounds.In the course of searching for biologically active substances from marine sources,we chemically investigated the metabolites of fungi Dendrodochium.sp.(internal strain no.10087) and Phialemonium.sp.(internal strain no.10082) associated with the sea cucumberHolothuria nobilis Selenka, collected from the South China Sea.32compounds wereisolated by using a variety of modern chromatographic techniques, including normal phasesilica gel, reverse phase silica gel and Sephadex LH-20, and HPLC. Structures of27compounds were elucidented on the basis of modern spectroscopic method, includingNMR、HRMS、CD、UV、IR. Absolurte configuration of some compounds were determinedby TDDEFT/CD calculation and modified Mosher’s methods. These compounds can besorted into three types, namely polyhydroxy cyclohexanols,12-membered microlactone,and benzene derivatives.Compounds isolated from fungi associated with the sea cucumber Holothuria nobilisSelenka were obtained in vitro anti-fungal activity, results show that the screening ofanti-fungal compounds have a certain activity. Also did antitumor activity, showed havecertain activity to MG-63cell and A549cell, but no activity to Lovo cell. Our studiesfocused on chemical constituents of the fungi Dendrodochium.sp. and Phialemonium.sp.and their bioactivities have established a foundation for further research, and providedimportant leading compounds for the development of new antifungal drugs.