Studies Of The Risk Factors And The Snps Of The Urate Transporters Associated With The Patients Suffering From Gouty Arthritis In Chinese Han Population

Objection: Gout are a series of syndromes caused by the over-soluble monosodiumurate monohydrate (MSU) deposits in somewhere of the body, including gouty arthritis,gouty renal disease, gouty renal calculus, tophus, et al. It is a dominant representation ofgout that acute gouty arthritis recurs time after time. The attack involving the firstmetatarsophalangeal joints most is serious and the course is confined by itself. The exactpathogenesis of gout is still undiscovered. At present it is clear that gout may be associatedwith hyperuricemia. The objections of the research aim to find some risk factors that mayresult in gout through usual physical examination and explore the genetic pathogenesis ofgout through the analyses of SNPs of several urate transporters. Thus it may be discoveredthat risk factors that might induce gout through physical examination or gene detection andvaluable measures may be taken to prevent gout from attack.Method: The research on the risk of acute attack of a gouty arthritis introduces a1：1matched case-control study.104patients with an acute attacked gout were recruited as thepatients group and104healthy people with matched age and gender as the controlledgroup. The general data such as the height, the body mass, the blood pressure, and so on, ofthe two groups were written down and the blood biochemistry tests of the two groups weretaken and the reports were marked down. Multivariate logistic regression analysis wasadopted to the research of the rules of the data. As to the analyses of SNPs of several uratetransporters the author took the following steps. Firstly, the blood specimens wereextracted from a cohort of204Chinese Han population including102patients sufferingfrom acute gouty arthritis and102healthy matched persons. Secondly, the DNA wasextracted from the blood specimens using special kits. Finally, the SNP(Single NucleotidePolymorphisms) scan was performed at ten loci of eight genes that express uratetransporters protein.Result: The major age of the first acute attack of gout is in a man’s forties. Thedifference between the gender is obvious for the male gout versus the female gout is16：3.Uric acid，total cholesterol，triglyceride and diastolic blood pressure are the four factorsthat influence the acute attack of gout. The respective relative risk is29.3，25.7，10.2and3.3. Of all the SNPs detected, rs2231142in ABCG2expressed three types as G/G, G/T andT/T. The corresponding data in the patients group were23,42,37and the counterpart inthe healthy were53,40,9. The data showed statistic significance. It was also found that A/A gene at the rs6855911of GLUT9in the gouty patients was4.122times in risk levelmore than that in the healthy people. Other SNPs failed the significance level.Conclusion：A man in his forties is easily attacked by gout. Hyperuricemia，highblood level of total cholesterol，triglyceride and diastolic blood pressure are the four riskfactors for acute attack of gout． The locus rs2231142which is located in gene ABCG2with an expression of T/T genetype could be associated with gout while with an expressionof G/G genetype could protect the person from gout attack. A person with the locusrs6855911which is located in gene GLUT9expressing A/A genetype could be susceptibleto suffer from gout.