Research On The Isolation Of Endophytic Fungi From Tea Trees In Cangshan Mountain System And Its Antibacteria Activity

【Objectives】The problem will take the Da Li tea trees growing in Cang Shan mountainsystem as object of study, will optimizes out the separation condition being fit to owekind of plant endogenous fungus; And then, separate out endogenous fungus and carryout the bacterium species appraisal; Secondly, abstract out the culture outcomeseparating the fungus to endogeny; Come to do the bacteriostat experiment finally,with picking up a thing, seek the endogenous fungus having a bacteriostasis.【Methods】Collect a total of18samples from older leaf,tender leaf,branch in Da Liacademy tea garden, GanTong tea garden, the Tai He village tea garden of Cang Shanmountain system, trying to find out out endogenous fungus separation condition, bebased on the separation, purification owing condition the endogenous fungus being inprogress again, carrying out the bacterium species appraisal with ITS array analysismethod combining with a morphology, use cell to break law to pieces extractingdrawing following the outcome carrying out a supersession combining with anorganic solvent and then including that surface sterilisatio condition and cultivatescondition, Dilution law makes the supersession outcome bacteriostat experimentfinally with tiny unnatural bouillon, detecting puts up and the minimal bacteriostatthickness having bacteriostasis bacterial strain.【Results】1.Have optimized out the separation condition being suitable to endogenousfungus of Cang Shan county mountain chain tea garden tea plant: Surface of tea plantstem sterilisatio condition alcohol sterilisatio for75%2mins,5.2%’s hypochlorousacid sodium sterilisatio5minutes, sterile water of queen potch four time, the culturemedium uses the PDA culture medium; Surface of tea leaf sterilisatio condition alcohol sterilisatio for75%2minutes,5.2%’s hypochlorous acid sodium sterilisatio20seconds, sterile water of queen potch four time, the culture medium uses the PDAculture medium；2.18portion of sample separates out13bacterial strains：GTY1、GTY2、GTL1、GTL2、GCS1、GCS2、GCL1、GCL2、GCL3、GCL4、GCL5、GCY1、GCY2.3. Endogenous13fungus has identified out the others12, being an unknownbacterium. Endogenous12fungus is hit by8(GTY2, GCS1, GCS2, GCL3, GCL4,GCL5, GCY1, GCY2)(Colletotrichum gloeosporioides) in order the thorn set sporeis checked the spore bacterium by orange thorn, that2are a Kong Qiu of secretlystraightforward spore of straightforward thicket spore eye section spore (GTY1,GCL2) is, that1are that secretly straightforward spore of straightforward thicketspore eye section saves the water chestnut spore (GTL1) is, that1are that black plateof black set spore eye spore section checks the crinosity spore (GCL1) is.4. Test the fungus having sifted out4endogeny having a bacteriostasis: GCL1,GCL4, GCL5, GTY2. GCL1, GCL4are worth difference to the bacillus coli MIC inorder32mug/ml compose in reply8mug/ml; GCL1, GCL4, GCL5are worth to thestaphylococcus aureus MIC in order32mug/ml,32mug/ml compose in reply2mug/ml; GTY2is8mug/ml to MIC of green pus bacillus value.【Conclusion】1. The endogenous tea plant fungus separation condition that the experimentoptimizes out is fit to owe kind of plant material;2. The fungus community structure studying the tea plant finally indicating theCang Shan county mountain chain diversity tea garden is endogenous originally isdifferent, the endogenous fungus bacterium species has and diversity composing inreply rich；3. In the endogenous fungus parting for out, the thorn set spore category orangethorn plate spore bacterium (Colletotrichum gloeosporioides) is an advantagebacterium species；4. There are4plants (GCL1, GCL4, GCL5, GTY2) have the bacteriostasisbacterial strain of experiment preparation by screening take place；5. Provided the experimental basis for the application of research Cangshan teaEndophytic fungi。...