Research Of Preparation And Application Of Liposomes

Since the liposomes were found, they showed a good prospect in dragcarrier because they could be gegraded in vivo without toxicity; they were alsotaken seriously by scholars and pharmaceutical companies because oftargeting and reduced toxicity.In this study, we optimized and found up the gentiopicroside liposomes’model, and we also studied the application of liposomes influorescently-labeled:(1) We studied the multiple emulsion method, thin-film hydration method,reverse phase evaporation method. And we finaly chosen the multipleemulsion method as the preparation method.(2) We used single factor and Box-Behnken design to optimize theoptimization formulation as follows: the concentration of gentiopicroside was2.04mg/mL,the time of ultrasound during the first emulsification was7.07min,the quality rate of Chol: EPC was0.45mg/mg. Unde the formulation, theliposomes’ entrapment efficiency and drug loading capacity were52.39%and2.69%respectively, which were less than1%compared with the predictedvalue. (3) The solution of gentiopicroside liposomes showed a blue opalescence,and the diameters was131nm, zeta potential was-38.77mV. The liposomeswere regular single-cavity sphere under TEM, the phase transition temperaturewas25℃which measured by DSC, and the oxidation index was0.1608. Andwe found that the moist heat sterilization method was better than thefreeze-dried and sterilized by ultraviolet method during the study.(4) The ZnS fluorescent quantum dots-liposomes which prepared by thestudy had a maximum fluorescence peak around360nm when be excitedunder280nm.