An Experimental Study On The Nerve Repair Mechanisms Of Schwann Cells Modified With Glia Derived Neurotrophic Factor On Spinal Cord Injury

Objective: To explore the protective effect of the GDNF gene transfected into Schwanncells of spinal cord injury.Methods:200rats were randomly divided into five groups (n=40). Use MASCIS(Multicenter Animal Spinal Cord Injury Study) Impactor Model-II blow device made ofspinal cord injury model,divided into normal group; laminectomy+injury group;laminectomy+injury+not transfected Schwann cells group; vertebral board resection+injury+empty vector transfected Schwann cell group; laminectomy+injury+pEGFP-N1/GDNF transfected Schwann cell group. Postoperative start to one day after fiveweeks using the BBB score for hindlimb recovery rate to randomly selected20rats from eachgroup for per week;at the same time the first postoperative day,7days and15days in eachgroup the remaining20rats by HE staining and by RT-PCR detection of Bax and Bcl-2expression, to determine the protective effect of GDNF gene modified Schwann cells of spinalcord injury.Results: The GDNF gene modified Schwann cells through reducing expression of theBax mRNA and improve expression of the Bcl-2mRNA.Then prove the treatment nerverepair mechanisms of the GDNF gene modified Schwann cells to spinal cord injury.Conclusion: GDNF gene modified Schwann cells of spinal cord injury has a nerverepair effect,and provide an experimental basis for GDNF genetically modified SCs treatmentof spinal cord injury on the cellular and molecular levels.