The Application And Comparation Of The Methods Of Drug-related Gene Detection In Tumor Personalized Medicine

Human diseases, especially the major common diseases, such as cardiovascular disease, psychiatric disorders, endocrine system diseases, the diagnosis, treatment results and prognosis of tumors, etc., in clinical practice there is a huge differences between population and individuals. Individualized treatment will be the new direction and an inevitable trend in the clinical diagnosis of disease and treatment, is one of the key directions for the21st century biomedical. Pharmacogenomics the basis of research and implementation of individualized treatment, there are many ways for the detection of gene. Each method has its own characteristics and advantages, there are also varying degrees of limitations in the application. This topic we selected three different detection methods for a few anticancer drugs related genes to conduct applied research.We apply the gap-ligase chain reaction (Gap-LCR) to detect seven gene polymorphism of the CYP2D6gene in10cases at first, The test results showed that the method good of application. Use other methods to validate the results at the same time, further verify the operability and reliability of Gap-LCR. Gap-LCR assay detect gene mutation in the cost low and reproducible, and require relatively simple equipment. SNP detection can be carried out under limited laboratory conditions, which is very suitable for clinical promotion.Sanger sequencing is the.gold standard to determine the mutation and can read out the sequence directly. We apply the Sanger sequencing method to detect the gene mutations which related targeted cancer therapy drug, including K-ras, EGFR, C-kit, and PDGFRA etc., Sanger sequencing detected tumor targeting treatment-related gene mutations, results are intuitive and reliable, but some low gene mutations (<20%) can not be detected because of the carcinoid tumor heterogeneity and the limitations of sample source organization. In addition, we optimize a method to detect the sequences in a high GC content on the basis of the method detect common sequence.In this study, we use high-resolution melting curve (HRM) to detect the gene polymorphism of the fluoropyrimidine drug efficacy and toxicity of the predicted genes, which related the DPYD and TYMS gene. This Method successfully established, provides a new option for the genetic testing of the fluoropyrimidine drug efficacy and toxicity prediction genes.Compared with the previous detection methods, the genotyping method have an advantage because of low-cost, quick and easy, high sensitivity. We have also established a positive quality control system of the two polymorphisms of the DPYD gene, provides a basis forthe analysis of test results of unknown samples.