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The Vitro Antioxidant Activity And Total Phenols Composition Of The Extraction Process Of The Typhonium Giganteum Engl

Posted on:2013-10-01Degree:MasterType:Thesis
Country:ChinaCandidate:Z SongFull Text:PDF
GTID:2234330374472686Subject:Medicinal chemistry
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The Typhonium giganteum Engl. has a long history of TCM drugs and a wide range of pharmacological activity. In this paper, heat reflux extraction, ultrasonic assisted extraction and negative pressure cavity extraction from typhonium giganteum Engl is investigated by DPPH method. ABTS method and FRAP in vitro antioxidant activity.In order to research the antioxidant components of Typhonium,we use the forint phenol method and nitrite aluminum-sodium hydroxide chromogenic method to measure the polyphenol and total flavonoid content of the three extractions. The results show that the extraction of three methods has been significant in vitro antioxidant activity. The DPPH radical scavenging rate reached above90%. Relative antioxidant activity of ABTS was0.59mM/g,0.45mM/g,0.12mM/g. FRAP total antioxidant capacity values were1.26mM/mg,1.17mM/mg,1.01mM/mg. The polyphenol content and the total flavonoids content of three extractions are31.4%,26.24%,28.16%and3.42%,2.99%,2.98%.In order to more rational development and utilization of medicinal plant resources in the typhonium,comprehensive consideration of the above experimental results, we select the extraction typhonium tubers total phenolics effect better thermal reflux method for the optimization of the extraction process parameters.Single factor to determine the solvent solid-liquid ratio and extraction times.Using response surface methodology to study the concentration of the extraction solvent, extraction time and temperature. Extract Typhonium tubers in the total phenolic composition of the optimum parameters of the thermal reflux method is:Solid-liquid ratio of1:20; ethanol concentration of71%; extraction time is95minutes; extraction temperature to59℃; three times the number of extraction. In those conditions, extracting the total phenolic components from Typhonium tubers is maximum.
Keywords/Search Tags:Typhonium giganteum Engl., Antioxidant, Total phenols, Processoptimization
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